LEMD3 Protein (AA 2-911) (rho-1D4 tag)

Catalog No. ABIN3093639

Product Details

Target: LEMD3 (LEM Domain Containing 3 (LEMD3))

Protein Type: Recombinant

Protein Characteristics: AA 2-911

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This LEMD3 protein is labelled with rho-1D4 tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: AAAAASAPQQ LSDEELFSQL RRYGLSPGPV TESTRPVYLK KLKKLREEEQ QQHRSGGRGN KTRNSNNNNT AAATVAAAGP AAAAAAGMGV RPVSGDLSYL RTPGGLCRIS ASGPESLLGG PGGASAAPAA GSKVLLGFSS DESDVEASPR DQAGGGGRKD RASLQYRGLK APPAPLAASE VTNSNSAERR KPHSWWGARR PAGPELQTPP GKDGAVEDEE GEGEDGEERD PETEEPLWAS RTVNGSRLVP YSCRENYSDS EEEDDDDVAS SRQVLKDDSL SRHRPRRTHS KPLPPLTAKS AGGRLETSVQ GGGGLAMNDR AAAAGSLDRS RNLEEAAAAE QGGGCDQVDS SPVPRYRVNA KKLTPLLPPP LTDMDSTLDS STGSLLKTNN HIGGGAFSVD SPRIYSNSLP PSAAVAASSS LRINHANHTG SNHTYLKNTY NKPKLSEPEE ELLQQFKREE VSPTGSFSAH YLSMFLLTAA CLFFLILGLT YLGMRGTGVS EDGELSIENP FGETFGKIQE SEKTLMMNTL YKLHDRLAQL AGDHECGSSS QRTLSVQEAA AYLKDLGPEY EGIFNTSLQW ILENGKDVGI RCVGFGPEEE LTNITDVQFL QSTRPLMSFW CRFRRAFVTV THRLLLLCLG VVMVCVVLRY MKYRWTKEEE ETRQMYDMVV KIIDVLRSHN EACQENKDLQ PYMPIPHVRD SLIQPHDRKK MKKVWDRAVD FLAANESRVR TETRRIGGAD FLVWRWIQPS ASCDKILVIP SKVWQGQAFH LDRRNSPPNS LTPCLKIRNM FDPVMEIGDQ WHLAIQEAIL EKCSDNDGIV HIAVDKNSRE GCVYVKCLSP EYAGKAFKAL HGSWFDGKLV TVKYLRLDRY HHRFPQALTS NTPLKPSNKH MNSMSHLRLR TGLTNSQGSS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LEMD3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LEMD3 (LEM Domain Containing 3 (LEMD3))

Alternative Name: LEMD3 (LEMD3 Products)

Synonyms: MAN1 Protein, AI316861 Protein, Man1 Protein, im:7158446 Protein, wu:fb34b12 Protein, si:dkey-6a5.6 Protein, RGD1561001 Protein, man1 Protein, sane Protein, xman1 Protein, lemd3 Protein, LEM domain containing 3 Protein, LEM domain containing 3 S homeolog Protein, LEMD3 Protein, Lemd3 Protein, lemd3 Protein, lemd3.S Protein

Background: Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest. {ECO:0000269|PubMed:15601644, ECO:0000269|PubMed:15647271}.

Molecular Weight: 101.0 kDa Including tag.

UniProt: Q9Y2U8