EXOC3 Protein (AA 1-755) (Strep Tag)

Catalog No. ABIN3123527

Product Details

Target: EXOC3 (Exocyst Complex Component 3 (EXOC3))

Protein Type: Recombinant

Protein Characteristics: AA 1-755

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This EXOC3 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MCKDSACFLT MKETDLEAVA TAVQRVAGML QRPDQLDKVE QYRRREARKK ASVEARLKAA IQSQLDGVRT GLSQLHNALN DVKDIQQSLA DVSKDWRQSI NTIESLKDVK DAVVQHSQLA AAVENLKNIF SVPEIVRETQ DLIEQGALLQ AHRKLMDLEC SRDGLMCEQY RMDSGNKRDM TLIHGYFGST QGLSDELAKQ LWMVLQRSLV TVRRDPTLLV SVVRIIEREE KIDRRILDRK KQTGFVPPGR PKNWKEKMFA ILDRTVTTRI EGTQADTRES DKMWLVRHLE IIRKYVLDDL IVAKNLMVQC FPPHYEIFKN LLSMYHQALS TRMQDLASED LEANEIVSLL TWVLNTYTSA EMMGNVELAP EVDVSALEPL LSPNIVSELL DTYMSTLTSN IIAWLRKALE TDKKDWSKET EPEADQDGYY QTTLPAIVFQ MFEQNLQVAA QISEDLKTKV LVLCLQQMNS FLSRYKDEAQ LYKEEHLRNR QHPHCYVQYM IAIINNCQTF KESIISLKRK YLKTEAEEGL CLSQPSMDGI LDAIAKEGCS SLLEEVFLDL EQHLNELMTK KWLLGSNAVD IICVTVEDYF NDFAKIKKPY KKRMTAEAHR RVVVEYLRAV MQKRISFRSA EERKEGAEKM VREAEQLRFL FRKLASGFGE DADGHCDTIV AVAEVIKLTD PSLLYLEVST LVSKYPDIRD DHIGALLALR GDASRDMKQT IMETLEQGPM QASPNYVPIF KEIVVPSLNV AKLLK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EXOC3 (Exocyst Complex Component 3 (EXOC3))

Alternative Name: Exoc3 (EXOC3 Products)

Synonyms: CG5341 Protein, Dmel\\CG5341 Protein, Dsec6 Protein, Sec6 Protein, Sec6p Protein, dsec6 Protein, sec 6 Protein, F14O23.20 Protein, F14O23_20 Protein, sec6l1 Protein, fi26g09 Protein, wu:fi26g09 Protein, wu:fi34a09 Protein, wu:fj62h05 Protein, wu:fk66f08 Protein, zgc:55709 Protein, SEC6L1 Protein, SEC6 Protein, 2810050O03Rik Protein, E430013E20Rik Protein, Sec6l1 Protein, rSec6 Protein, Secretory 6 Protein, SEC6 Protein, exocyst complex component 3 Protein, Exocyst complex component 3 Protein, Sec6 Protein, SEC6 Protein, EXOC3 Protein, exoc3 Protein, Exoc3 Protein, sec-6 Protein

Background: Exocyst complex component 3 (Exocyst complex component Sec6),FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. {ECO:0000250}.

Molecular Weight: 86.5 kDa

UniProt: Q6KAR6

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis