TTLL1 Protein (AA 1-423) (Strep Tag)

Catalog No. ABIN3127253

Product Details

Target: TTLL1 (Tubulin tyrosine Ligase-Like Family, Member 1 (TTLL1))

Protein Type: Recombinant

Protein Characteristics: AA 1-423

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TTLL1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAGRVKWVTD IEKSVLINNF EKRGWIQVTE NEDWNFYWMS VQTIRNVFSV ETGYRLSDDQ IVNHFPNHYE LTRKDLMVKN IKRYRKELEK EGSPLAEKDE NGKYLYLDFV PVTYMLPADY NLFVEEFRKS PSSTWIMKPC GKAQGKGIFL INKLSQIKKW SRDSKTSSFV SQSTKEAYVI SVYINNPLLI GGRKFDLRLY VLVSTYRPLR CYMYKLGFCR FCTVKYTPST SELDNMFVHL TNVAIQKHGE DYNHIHGGKW TVNNLRLYLE STRGREVTSK LFDEIHWIIV QSLKAVAPVM NNDKHCFECY GYDIIIDDKL KPWLIEVNAS PSLTSSTAND RILKYNLIND TLNIAVPNGE IPDCKWNKSP PKEVLGNYEI LYDEELAQGD GAERELRNRP GQPVGPRAGR SRDSGRSVLT TWK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TTLL1 (Tubulin tyrosine Ligase-Like Family, Member 1 (TTLL1))

Alternative Name: Ttll1 (TTLL1 Products)

Synonyms: 6330444E16Rik Protein, AV014541 Protein, C22orf7 Protein, HS323M22B Protein, zgc:63993 Protein, tubulin tyrosine ligase-like 1 Protein, tubulin tyrosine ligase like 1 Protein, tubulin tyrosine ligase-like family, member 1 Protein, Ttll1 Protein, TTLL1 Protein, ttll1 Protein

Background: Polyglutamylase complex subunit TTLL1 (EC 6.3.2.-) (Tubulin polyglutamylase TTLL1) (Tubulin polyglutamylase complex subunit 3) (PGs3) (Tubulin--tyrosine ligase-like protein 1) (p49),FUNCTION: Catalytic subunit of a polyglutamylase complex which modifies tubulin, generating side chains of glutamate on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin (PubMed:15890843). Probably involved in the side-chain elongation step of the polyglutamylation reaction rather than the initiation step (Probable). Modifies both alpha- and beta-tubulins with a preference for the alpha-tail (PubMed:15890843, PubMed:22170066). Unlike most polyglutamylases of the tubulin--tyrosine ligase family, only displays a catalytic activity when in complex with other proteins as it is most likely lacking domains important for autonomous activity (PubMed:15890843). Part of the neuronal tubulin polyglutamylase complex (PubMed:15890843). Mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility (PubMed:20498047, PubMed:20442420, PubMed:23897886). Involved in respiratory motile cilia function through the regulation of beating asymmetry (PubMed:20498047, PubMed:20442420). Essential for sperm flagella biogenesis, motility and male fertility (PubMed:20442420). Also mediates glutamylation of non-tubulin proteins (PubMed:29593216). Involved in KLF4 glutamylation which impedes its ubiquitination, thereby leading to somatic cell reprogramming, pluripotency maintenance and embryogenesis (PubMed:29593216). {ECO:0000269|PubMed:15890843, ECO:0000269|PubMed:20442420, ECO:0000269|PubMed:20498047, ECO:0000269|PubMed:22170066, ECO:0000269|PubMed:23897886, ECO:0000269|PubMed:29593216, ECO:0000305|Ref.11}.

Molecular Weight: 49.1 kDa

UniProt: Q91V51