MORC3 Protein (AA 1-942) (Strep Tag)

Catalog No. ABIN3131195

Product Details

Target: MORC3 (MORC Family CW-Type Zinc Finger 3 (MORC3))

Protein Type: Recombinant

Protein Characteristics: AA 1-942

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MORC3 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MAAQPPTGIR LSALCPKFLH TNSTSHTWPF SAVAELIDNA YDPDVNAKQI WIDKTVISDH ICLTFTDNGN GMTADKLHKM LSFGFSDKVT MNGHVPVGLY GNGFKSGSMR LGKDAMVFTK NGETMSVGFL SQTYLEVIKA EHVVVPIVTF NKHRQMINLT ESKASLAAIL EHSLFSTEQK LLAELNAIMG KKGTRIIIWN LRSYKNATEF DFEKDKYDIR IPEDLDETAG RKGYKKQERM DQIAPESDYS LRAYCSILYL KPRMQIIIRG QKVKTQLVSK SLAYIERDVY RPKFLTRTVR ITFGFNCRNK DHYGIMMYHK NRLIKAYEKV GCQLKANNMG VGVVGIIECN FLKPTHNKQD FDYTNEYRLT ILALGEKLND YWNEMKVKKN AEYPVNLPVE DIQKRPDQTW VQCDACLKWR KLPDGIDQLP EKWYCSNNPD PQFRNCEVPE EPEDEDLVHP TYEKTYKKTS KERFRIRQPE ILPRILPQIN PELLYQTSVS SQSFSPVKES VPRPHLSEVT SPFAARIINL NLASPASEPE NSSMKRKLGV HSSILNAKTR RLSNPPVENS SYKNDDDEDV IILEENSTPK PAVDLEVKSD IEVKSEQSHT EQSGIHVDLV SSPKPCVQAS STSTSTSRSD PGITVSTQTD APGLTVKKEE SMEEDMGVRN GTATLSCVGT EAKVQETSAE SVDATSHQLQ ELRSELLVVT QERDDYKRQC QMFTDQIQVL QQRLLEMNDK CVKKEKCHQS TETDAVFLLD SVNGQAESLD HLGSQYQQAL QEIERLKRQC SALQQVKSEC SQASCTESKS EVDEMAVQLD DVFRQLDKCT IERDQYKNEV QLLEIEKSHI HSQCEELQTE VEQLKSTGQQ AAADGSTASN AEEPVSYVDG ESLKLRSLRV NVGQLLAMIV PDLDLQQVNY DVDVVDEILG QVVEQMSEIS ST
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MORC3 (MORC Family CW-Type Zinc Finger 3 (MORC3))

Alternative Name: Morc3 (MORC3 Products)

Synonyms: NXP2 Protein, ZCW5 Protein, ZCWCC3 Protein, 1110051N18Rik Protein, AI452146 Protein, BF318192 Protein, D16Jhu32e Protein, Zcwcc3 Protein, sb:cb561 Protein, sb:cb69 Protein, zgc:101052 Protein, fb73c08 Protein, wu:fb73c08 Protein, zgc:162471 Protein, morc3 Protein, MORC family CW-type zinc finger 3 Protein, microrchidia 3 Protein, MORC family CW-type zinc finger 3b Protein, MORC family CW-type zinc finger 3a Protein, MORC family CW-type zinc finger 3 S homeolog Protein, MORC3 Protein, Morc3 Protein, morc3b Protein, morc3a Protein, morc3.S Protein

Background: MORC family CW-type zinc finger protein 3 (Nuclear matrix protein 2) (Zinc finger CW-type coiled-coil domain protein 3),FUNCTION: Nuclear matrix protein which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism and plays a role in innate immunity by restricting different viruses through modulation of the IFN response. Mechanistically, possesses a primary antiviral function through a MORC3-regulated element that activates IFNB1, and this function is guarded by a secondary IFN-repressing function. Sumoylated MORC3-NBs associates with PML-NBs and recruits TP53 and SP100, thus regulating TP53 activity (PubMed:17332504). Binds RNA in vitro. Histone methylation reader which binds to non-methylated (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and trimethylated (H3K4me3) 'Lys-4' on histone H3. The order of binding preference is H3K4me3 > H3K4me2 > H3K4me1 > H3K4me0. {ECO:0000250|UniProtKB:Q14149, ECO:0000269|PubMed:17332504}.

Molecular Weight: 106.5 kDa

UniProt: F7BJB9

Pathways: Maintenance of Protein Location