Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 Interacting Protein (NFATC2IP) (AA 1-412) protein (His tag)

Details for Product No. ABIN3131403, Supplier: Log in to see
Protein Name
  • wu:fj84d09
  • NFATC2IP
  • D7Ertd304e
  • NIP45
  • ESC2
  • RAD60
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein
  • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 interacting protein
  • nfatc2ip
  • NFATC2IP
  • Nfatc2ip
Protein Characteristics
AA 1-412
1
1
1
1
1
1
Origin
Mouse (Murine)
2
1
1
1
Source
Insect Cells
3
1
1
Protein Type
Recombinant
Purification tag / Conjugate
His tag
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence MAEPLRGRGP RSRGGRGARR ARGARGRCPR ARQSPARLIP DTVLVDLVSD SDEEVLEVAD PVEVPVARLP APAKPEQDSD SDSEGAAEGP AGAPRTLVRR RRRRLLDPGE APVVPVYSGK VQSSLNLIPD NSSLLKLCPS EPEDEADLTN SGSSPSEDDA LPSGSPWRKK LRKKCEKEEK KMEEFPDQDI SPLPQPSSRN KSRKHTEALQ KLREVNKRLQ DLRSCLSPKQ HQSPALQSTD DEVVLVEGPV LPQSSRLFTL KIRCRADLVR LPVRMSEPLQ NVVDHMANHL GVSPNRILLL FGESELSPTA TPSTLKLGVA DIIDCVVLAS SSEATETSQE LRLRVQGKEK HQMLEISLSP DSPLKVLMSH YEEAMGLSGH KLSFFFDGTK LSGKELPADL GLESGDLIEV WG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Nfatc2ip Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Background In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter, this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression. Down-regulates formation of poly-SUMO chains by UBE2I/UBC9. {ECO:0000269|PubMed:20077568, ECO:0000269|PubMed:20133688}.
Molecular Weight 46.1 kDa Including tag.
UniProt O09130
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 Interacting Protein (NFATC2IP) (AA 1-412) protein (His tag) (ABIN3131403) „Crystallography Grade“ protein due to multi-step, protein-specific purification process