APOBR Protein (AA 1-942) (Strep Tag)

Catalog No. ABIN3136689

Product Details

Target: APOBR (Apolipoprotein B Receptor (APOBR))

Protein Type: Recombinant

Protein Characteristics: AA 1-942

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This APOBR protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MDFLRLRLPG LHQALRGALD SFSAFVSYLV GDTVPTVERQ TQAAEELGEV TEGKVVGEEA QEVLEGLRSG QSEGVEAPEE TRRCQEGSLA GEQTWGWRAD SSARPQAERQ DTGSWKAAED ARGQEPSVPL KPEAEPGTHR DRSSNTAQEI WEHGEEEASS GEPLRTCEQK EEEEEVVRAA ESGMAEGVES QPTWHSEPGG NAGTEGQHVT EDSKEIDWVA KDMVAEIEWF GAKGIDKEEE RMVPMRDGER ARAQGTQCPG AESEDQAMLS REAWTVSDRE GADSLGVQGT EYGSDPGDNF PGTTGRVWVL EEADKGDQQD EVDEKREAEV RFPIQTLEAE RTGEMTEGHI AEEEAMGEQE TEGSFEDEER QDLAIRDNGV SLEEEVRAEE SSREKRNSWA TEPTLVLDTE AKDEPDWEDS PEVSTEELFV GERSEAAQMT PEVLRVKVTE GQDPELVRHS QALTKQLEEG QKGQEETSGA PDLSPERVLS LKEYPGPVGF AGPELEAWGN WSRGVDRRNS QEVKADAEAG KEQTATEQAV EIRAEGGQEA QQPEVFGSGG EEALTSVALN PELEGSQGAE AGTEESVEES KPTENEAAEE EAVVPWEADG TCRKRRLEEV TLSLQDSEDT ETSYLAEEII VGIRAVDTEE GPKWEAGLAP ETELGKAWCS EGRGEAGRGT ELEETTEKQS GQEVGLVGSA EKVSGYDIQE IDGTEEGEQA EMETSVMAED IRGTDGVTLG SQAERAEGSI TPMETEGLLR DQMLLEEEAG GGQSREQKVH NSEGEIQTLD DSSDQEGQQT HQIPTVAVPG PLESAEATAG APGDVHSNWN EALLPGSRLD VSVPRSRVLL SRSSSRRRSR PSFHRISVPE PQCDPPSPQP QAERPVPEQS SLQLEETPEL SATKPEGTPV PARRKMLGRG FGFAHPGMMQ ELQARLSQPK PQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: APOBR (Apolipoprotein B Receptor (APOBR))

Alternative Name: Apobr (APOBR Products)

Synonyms: APOB48R Protein, Apob-48r Protein, Apob48r Protein, RGD1565169 Protein, APOB100R Protein, apolipoprotein B receptor Protein, apolipoprotein B48 receptor Protein, APOBR Protein, LOC745786 Protein, Apobr Protein

Background: Apolipoprotein B receptor (Apolipoprotein B-100 receptor) (Apolipoprotein B48 receptor) (apoB-48R),FUNCTION: Macrophage receptor that binds to the apolipoprotein B48 (APOB) of dietary triglyceride (TG)-rich lipoproteins (TRL) or to a like domain of APOB in hypertriglyceridemic very low density lipoprotein (HTG-VLDL). Binds and internalizes TRL when out of the context of the macrophage. May provide essential lipids to reticuloendothelial cells. Could also be involved in foam cell formation with elevated TRL and remnant lipoprotein (RLP). Mediates the rapid high-affinity uptake of chylomicrons (CM), HTG-VLDL, and trypsinized (tryp) VLDL devoid of APOE in vitro in macrophages. {ECO:0000269|PubMed:12177162}.

Molecular Weight: 102.7 kDa

UniProt: Q8VBT6