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IVNS1ABP Protein (AA 1-642) (Strep Tag)

Crystallography grade IVNS1ABP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Catalog No. ABIN3136864
  • Target See all IVNS1ABP Proteins
    IVNS1ABP (Influenza Virus NS1A Binding Protein (IVNS1ABP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-642
    Origin
    Mouse
    Source
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This IVNS1ABP protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MIPNGYLMFE DENFIESSVA KLNALRKSGQ FCDVRLQVCG HEMLAHRAVL ACCSPYLFEI FNSDSDPHGV SHVKLDDLNP EAVEVLLNYA YTAQLKADKE LVKDVYSAAK KLKMDRVKQV CGDYLLSRMD VTSCISYRNF ASCMGDSRLL NKVDAYIQEH LLQISEEEEF LKLPRLKLEV MLEDNVCLPS NGKLYTKVIN WVQRSIWENG DSLEELMEEV QTLYYSADHK LLDGNPLDGQ AEVFGSDDDH IQFVQKKPPR ENGHKQISGS STGCLSSPNA SMQSPKHEWK IVASEKTSNN TYLCLAVLDS TFCVIFLHGR NSPQSSPTST PKLSKSLSFE MQPDELLEKP MSPMQYARSG LGTAEMNGKL IAAGGYNREE CLRTVECYDP HTDHWSFLAP MRTPRARFQM AVLMGQLYVV GGSNGHSDDL SCGEMYDPNI DDWTPVPELR TNRCNAGVCA LNGKLYIVGG SDPYGQKGLK NCDVFDPVTK SWTSCAPLNI RRHQSAVCEL GGYLYIIGGA ESWNCLNTVE RYNPENNTWT LIAPMNVARR GAGVAVLDGK LFVGGGFDGS HAISCVEMYD PTRNEWKMMG NMTSPRSNAG ITTVGNTIYA VGGFDGNEFL NTVEVYNPQS NEWSPYTKIF QF
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Grade
    Crystallography grade
    Top Product
    Discover our top product IVNS1ABP Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    IVNS1ABP (Influenza Virus NS1A Binding Protein (IVNS1ABP))
    Alternative Name
    Ivns1abp (IVNS1ABP Products)
    Synonyms
    1190004M08Rik Protein, 1700126I16Rik Protein, AA960440 Protein, HSPC068 Protein, ND1 Protein, NS-1 Protein, NS1-BP Protein, Nd1-L Protein, Nd1-S Protein, mKIAA0850 Protein, cb1052 Protein, fj23g11 Protein, ivns1abp Protein, wu:fj23g11 Protein, FLARA3 Protein, KLHL39 Protein, NS1BP Protein, fi13f08 Protein, fi41c09 Protein, wu:fi13f08 Protein, wu:fi41c09 Protein, influenza virus NS1A binding protein Protein, influenza virus NS1A binding protein a Protein, influenza virus NS1A binding protein L homeolog Protein, influenza virus NS1A binding protein b Protein, Ivns1abp Protein, ivns1abpa Protein, ivns1abp.L Protein, IVNS1ABP Protein, ivns1abpb Protein
    Target Type
    Influenza Protein
    Background
    Influenza virus NS1A-binding protein homolog (NS1-BP) (NS1-binding protein homolog) (Kelch family protein Nd1-L) (ND1-L2) (Nd1-S),FUNCTION: Involved in many cell functions, including pre-mRNA splicing, the aryl hydrocarbon receptor (AHR) pathway, F-actin organization and protein ubiquitination. Plays a role in the dynamic organization of the actin skeleton as a stabilizer of actin filaments by association with F-actin through Kelch repeats (PubMed:12213805, PubMed:16317045). Protects cells from cell death induced by actin destabilization (PubMed:16952015). Functions as a modifier of the AHR/Aryl hydrocarbon receptor pathway increasing the concentration of AHR available to activate transcription (By similarity). In addition, functions as a negative regulator of BCR(KLHL20) E3 ubiquitin ligase complex to prevent ubiquitin-mediated proteolysis of PML and DAPK1, two tumor suppressors (By similarity). Inhibits pre-mRNA splicing (in vitro) (By similarity). {ECO:0000250|UniProtKB:Q9Y6Y0, ECO:0000269|PubMed:12213805, ECO:0000269|PubMed:16317045, ECO:0000269|PubMed:16952015}., FUNCTION: [Isoform 2]: May play a role in cell cycle progression in the nucleus. {ECO:0000269|PubMed:15684717}.
    Molecular Weight
    71.6 kDa
    UniProt
    Q920Q8
    Pathways
    Negative Regulation of intrinsic apoptotic Signaling
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