LIMD1 Protein (AA 1-668) (Strep Tag)

Catalog No. ABIN3137371

Product Details

Target: LIMD1 (LIM Domains Containing 1 (LIMD1))

Protein Type: Recombinant

Protein Characteristics: AA 1-668

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This LIMD1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MDKYDDLGLE ASKFIEDLNM YEASKDGLFR VDKGAGNNPE FEETRRVFAT KMAKIHLQQQ QQQQLLQEEA LPRAGRSPVN GGNRQGASGK LAADGAAKPP LAVPTVAPGL ATTTAAAQPS YPSQEQRIRP SAHGARPGSQ NCGSREGPVS SQRPALHGLS PSCEDPSCLT HGDYYDNFSL ASPQWGDKPE GCPSVSLGVG SGWPGCPGND STLPKSCGDH HPYQPQLSTV CSGRSFESGI SGQDGGIGGH SSEKPTGLWS TASSQRVNLG FSSMGLENGT SAQPKGTTVS APMVPSSASQ GACPKRDSGL GYEASGRVFK PLVDTQPWLQ DGPKSYLSVS APLSSTAGKD STQPGMTTGL DPKFGCVESG TSPKPSPTSN VHPVMSTPSE LSCKESSPSW STDSSLEPVL PGSPTPSRVR LPCQTLAPGP ELGPSTAELK LEALTQRLER EMDAHPKADY FGSCVKCSKG VFGAGQACQA MGDLYHDACF TCAACSRKLR GKAFYFVNGK VFCEEDFLYS GFQQSADRCF LCGHLIMDMI LQALGKSYHP GCFRCVICNE CLDGVPFTVD SENKIYCVRD YHKVLAPKCA ACGLPILPPE GSDETIRVVS MDRDYHVECY HCEDCGLELN DEDGHRCYPL EDHLFCHSCH VKRLEKGPSP APLHQHHF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LIMD1 (LIM Domains Containing 1 (LIMD1))

Alternative Name: Limd1 (LIMD1 Products)

Synonyms: AW822033 Protein, D9Ertd192e Protein, xlimd1 Protein, LIM domains containing 1 Protein, LIM domains containing 1 L homeolog Protein, LIMD1 Protein, Limd1 Protein, limd1 Protein, limd1.L Protein

Background: LIM domain-containing protein 1,FUNCTION: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation. {ECO:0000269|PubMed:17092936, ECO:0000269|PubMed:18657804}.

Molecular Weight: 71.4 kDa

UniProt: Q9QXD8

Pathways: Ribonucleoprotein Complex Subunit Organization