CKM Protein (AA 1-381) (His tag)
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- Target See all CKM Proteins
- CKM (Creatine Kinase, Muscle (CKM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-381
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This CKM protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human CKM Protein is produced by our mammalian expression system and the target gene encoding Met1-Lys381 is expressed with a 6His tag at the C-terminus.
- Sequence
- MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSLLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ KVDHHHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product CKM Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10 % Glycerol, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- CKM (Creatine Kinase, Muscle (CKM))
- Alternative Name
- CKM (CKM Products)
- Synonyms
- CKMM Protein, M-CK Protein, Ckmm Protein, MCK Protein, ckmm Protein, m-ck Protein, CKM Protein, CK-M Protein, cb51 Protein, ckm Protein, mck Protein, wu:fa28d05 Protein, ckm3 Protein, wu:fb55e09 Protein, zgc:64204 Protein, zgc:92070 Protein, creatine kinase, M-type Protein, creatine kinase, muscle Protein, creatine kinase, M-type L homeolog Protein, creatine kinase, muscle a Protein, creatine kinase, muscle b Protein, CKM Protein, Ckm Protein, ckm.L Protein, ckm Protein, ckma Protein, ckmb Protein
- Gene ID
- 1158
- UniProt
- P06732
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