ACP1 Protein (AA 1-158) (His tag)

Catalog No. ABIN666750

This Recombinant ACP1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for ACP1 Protein (AA 1-158) (His tag) (ABIN666750)

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-158

Purification tag / Conjugate: This ACP1 protein is labelled with His tag.

Sequence: MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH

Characteristics: Acid phosphatase 1,1-158aa, Human, His-tagged, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 15,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM MES buffer ( pH 6.0) containing 0.1 mM PMSF, 2 mM EDTA, 10 % glycerol

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Alternative Name: LMW-PTP/ACP1

Background: Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Molecular Weight: 20.1 kDa (178aa) confirmed by MALDI-TOF

NCBI Accession: NP_009030