ACP1 Protein (GST tag)

Catalog No. ABIN7317711

Product Details

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ACP1 protein is labelled with GST tag.

Purpose: Recombinant Human ACP1/LMW-PTP Protein (GST Tag)(Active)

Sequence: Met 1-His 158

Characteristics: A DNA sequence encoding human ACP1 (AAI06012.1) (Met 1-His 158) was fused with the GST tag at the N-terminus.

Purity: > 88 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >65,000 pmol/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Alternative Name: ACP1/LMW-PTP (ACP1 Products)

Synonyms: HAAP Protein, 4632432E04Rik Protein, AI427468 Protein, Acp-1 Protein, LMW-PTP Protein, haap Protein, lmw-ptp Protein, ptp1a Protein, xlptp1 Protein, im:6910498 Protein, zgc:110844 Protein, Acp1 Protein, ACYPI006806 Protein, ACP Protein, ACPH Protein, APH Protein, Acph Protein, CG7899 Protein, Dmel\\CG7899 Protein, DDBDRAFT_0189318 Protein, DDBDRAFT_0266663 Protein, DDB_0189318 Protein, DDB_0266663 Protein, ACP1 Protein, XLPTP1 Protein, acp1 Protein, acp1a Protein, ptp1a-A Protein, LMW-PTPase Protein, acid phosphatase 1 Protein, acid phosphatase 1, soluble Protein, Acid phosphatase 1 Protein, acid phosphatase 1 L homeolog Protein, ACP1 Protein, Acp1 Protein, acp1 Protein, Acph-1 Protein, acp1.L Protein

Background:

Background: The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.

Synonym: HAAP,Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Adipocyte Acid Phosphatase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1, ACP1

Molecular Weight: 44.3 kDa