GM-CSF Protein (AA 18-141)
Quick Overview for GM-CSF Protein (AA 18-141) (ABIN667059)
Target
See all GM-CSF (CSF2) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 18-141
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Sequence
- MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
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Characteristics
- GM-CSF, 18-141 aa, Mouse, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is less or equal to 0.06ng/ml.
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein
CSF2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)CSF2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) protein (His tag)CSF2 Origin: Mouse Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 Origin: Rat Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 Origin: Human Host: HEK-293 Cells > 95 % AcA, CC Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) 10 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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Alternative Name
- GM-CSF
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Background
- GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Mouse GM-CSF protein is 125 amino acids and shares approximately 60 % homology with the human GM-CSF protein. Recombinant mouse GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer
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Molecular Weight
- 14 kDa (125aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_034099
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Pathways
- JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin
Target
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