Peroxiredoxin 1 Protein (PRDX1) (AA 1-199) (His tag)
Quick Overview for Peroxiredoxin 1 Protein (PRDX1) (AA 1-199) (His tag) (ABIN667075)
Target
See all Peroxiredoxin 1 (PRDX1) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-199
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Purification tag / Conjugate
- This Peroxiredoxin 1 protein is labelled with His tag.
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Sequence
- MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK
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Characteristics
- PRDX1, 1-199aa, Human, His tagged , Recombinant, E.coli
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Purification
- > 90% by SDS-PAGE
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Biological Activity Comment
- Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.
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Peroxiredoxin 1 (PRDX1) (AA 1-199) protein (GST tag)
PRDX1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Peroxiredoxin 1 (PRDX1) protein (His tag)PRDX1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 85 % as determined by SDS-PAGE
Peroxiredoxin 1 (PRDX1) (AA 1-199) (Active) protein (His tag)PRDX1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS, EAA Active
Peroxiredoxin 1 (PRDX1) (AA 1-199), (full length) protein (GST tag)PRDX1 Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % ELISA
Peroxiredoxin 1 (PRDX1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PRDX1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Peroxiredoxin 1 (PRDX1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PRDX1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 7.5) containing 20 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Peroxiredoxin 1 (PRDX1)
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Alternative Name
- Peroxiredoxin 1/PRDX1
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Background
- PRDX1 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein is also known to be important to protect red blood cells against reactive oxygen species and in tumor prevention. Recombinant human PRDX1, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
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Molecular Weight
- 24 kDa (219aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002565
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Pathways
- p53 Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
Target
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