ACAT2 Protein (AA 1-397) (His tag)
Quick Overview for ACAT2 Protein (AA 1-397) (His tag) (ABIN667155)
Target
See all ACAT2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-397
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Purification tag / Conjugate
- This ACAT2 protein is labelled with His tag.
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Sequence
- MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE
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Characteristics
- ACAT2, 1-397aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Acetyl-CoA Acetyltransferase 2 (ACAT2) (AA 1-397) protein (His tag)
custom-made ACAT2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Acetyl-CoA Acetyltransferase 2 (ACAT2) (AA 1-397) protein (His tag)custom-made ACAT2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Acetyl-CoA Acetyltransferase 2 (ACAT2) (AA 1-397) protein (GST tag)ACAT2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acetyl-CoA Acetyltransferase 2 (ACAT2) (AA 1-397) protein (His-IF2DI Tag)ACAT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 1 mM DTT, 10 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))
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Alternative Name
- ACAT2
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Background
- ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus. Recombinant human ACAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 45.4 kDa (433aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_005882
Target
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