ACAT2 Protein (AA 1-397) (His-TRX)

Catalog No. ABIN1692521

This Recombinant ACAT2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for ACAT2 Protein (AA 1-397) (His-TRX) (ABIN1692521)

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-397

Purification tag / Conjugate: This ACAT2 protein is labelled with His-TRX.

Purpose: Recombinant Human Sterol O-Acyltransferase 2/SOAT2/ACAT2 (N-Trx, 6His)

Sequence: MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM ADIGSMNAGS DPVVIVSAAR TIIGSFNGAL AAVPVQDLGS TVIKEVLKRA TVAPEDVSEV IFGHVLAAGC GQNPVRQASV GAGIPYSVPA WSCQMICGSG LKAVCLAVQS IGIGDSSIVV AGGMENMSKA PHLAYLRTGV KIGEMPLTDS ILCDGLTDAF HNCHMGITAE NVAKKWQVSR EDQDKVAVLS QNRTENAQKA GHFDKEIVPV LVSTRRGLIE VKTDEFPRHG SNIEAMSKLK PYFLTDGTGT VTPANASGIN DGAAAVVLMK KSEADKRGLT PLARIVSWSQ VGVEPSIMGI GPIPAIKQAV TKAGWSLEDV DIFEINEAFA AVSAAIVKEL GLNPEKVNIE GGAIALGHPL GASGCRILVT LLHTLERMGR SRGVAALCIG GGMGIAMCVQ RE

Characteristics: Recombinant Human Sterol O-acyltransferase 2 is produced by our E.coli expression system and the target gene encoding Met1-Glu397 is expressed with a Trx, 6His tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 um filtered solution of 20 mM Tris-HCl,100 mM NaCl,1 mM DTT,10 % Glycerol, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: minimize freeze-thaw cycles

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Alternative Name: ACAT2

Background: Alternative Names: Acetyl-CoA acetyltransferase cytosolic, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase, ACTL, ACAT2ACAT2 is a cytoplasmic enzyme which belongs to the thiolase family. ACAT2 takes part in lipid metabolism, lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. It is responsible for the synthesis of cholesteryl esters which are part of lipoproteins containing apoB. ACAT2 deficiency contributes to severe mental retardation and hypotonus.
Alternative Names: Acetyl-CoA acetyltransferase cytosolic, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase, ACTL, ACAT2

Molecular Weight: 59.1 kDa

UniProt: O75908