OGG1 Protein (AA 1-345) (His tag)

Catalog No. ABIN667178

This Recombinant OGG1 protein is produced in Escherichia coli (E. coli).

Quick Overview for OGG1 Protein (AA 1-345) (His tag) (ABIN667178)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-345

Purification tag / Conjugate: This OGG1 protein is labelled with His tag.

Sequence: MPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQR DYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRR KGSKGPEG

Characteristics: OGG1, 1-345aa, Human, His tag, E.coli

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 40 % glycerol

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: OGG1

Background: OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molecular Weight: 41.2 kDa (368aa) confirmed by MALDI-TOF

Pathways: DNA Damage Repair