ABO Protein (AA 54-354) (His tag)

Catalog No. ABIN667951

Product Details

Target: ABO (Histo-blood group A transferase (ABO))

Protein Type: Recombinant

Protein Characteristics: AA 54-354

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ABO protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: ABO, 54-354aa, Human, His tag, E.coli

Purity: > 85 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl

Storage: 4 °C

Target Details

Target: ABO (Histo-blood group A transferase (ABO))

Alternative Name: ABO (ABO Products)

Synonyms: A3GALNT Protein, A3GALT1 Protein, GTB Protein, NAGAT Protein, ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Protein, ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase, transferase B, alpha 1-3-galactosyltransferase) Protein, ABO Protein, Abo Protein

Background: ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Histo-blood group ABO system transferase, A3GALNT, A3GALT1, GTB, NAGAT. NCBI no.: NP_065202

Molecular Weight: 37.4kDa (322aa), confirmed by MALDI-TOF