Sonic Hedgehog Protein (SHH)
Quick Overview for Sonic Hedgehog Protein (SHH) (ABIN935799)
Target
See all Sonic Hedgehog (SHH) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
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Characteristics
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Purified recombinant Human Sonic Hedgehog protein
Expression System: E.coli
Bioactivity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 ?g/mL. -
Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) proteinSHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Mouse Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Sonic Hedgehog (SHH) (Active) proteinSHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Sonic Hedgehog (SHH) proteinSHH Origin: Human Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute to a concentration of 0.1-1.0 mg/mL.
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Buffer
- Supplied as a lyophilized powder. Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C to -80 °C for long term storage.
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- Sonic Hedgehog (SHH)
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Alternative Name
- Sonic Hedgehog
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Background
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Sonic the hedgehog homolog is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog and Indian hedgehog. SHH is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain.
Alternative Names: SHH protein -
Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Target
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