Surfactant Protein B Cross Reactive with Human Sp-B antibody
Reactivity: Pig
IA, WB
Host: Rabbit
Polyclonal
unconjugated
(2 references)-
- Target
- Surfactant Protein B Cross Reactive with Human Sp-B
- Reactivity
- Pig
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunoassay (IA), Western Blotting (WB)
- Sterility
- 0.2 μm filtered
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- Application Notes
- For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- Restrictions
- For Research Use only
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- Buffer
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- Expiry Date
- 12 months
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: "Quantitation of pulmonary surfactant protein SP-B in the absence or presence of phospholipids by enzyme-linked immunosorbent assay." in: Analytical biochemistry, Vol. 293, Issue 1, pp. 78-87, (2001) (PubMed).
: "Lipid-protein interactions of hydrophobic proteins SP-B and SP-C in lung surfactant assembly and dynamics." in: Pediatric pathology & molecular medicine, Vol. 20, Issue 6, pp. 445-69, (2001) (PubMed).
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: "Quantitation of pulmonary surfactant protein SP-B in the absence or presence of phospholipids by enzyme-linked immunosorbent assay." in: Analytical biochemistry, Vol. 293, Issue 1, pp. 78-87, (2001) (PubMed).
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- Target
- Surfactant Protein B Cross Reactive with Human Sp-B
- Background
- The polyclonal antibody recognizes the human surfactant protein B (SP-B). There are four surfactant- specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and
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