NS3 antibody

Catalog No. ABIN1169266

Product Details

Target: NS3

Reactivity: Hepatitis C Virus (HCV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Recognizes HCV NS3 (genotype 1a and 1b) (epitope mapped to NS3 aa 160-193), recognizes NS3 alone or the NS3-4A complex.

Cross-Reactivity: Hepatitis C Virus (HCV)

Cross-Reactivity (Details): Does not cross-react with HCV NS3 (genotype 2a).

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant hepatitis C virus (HCV) NS3 serine protease domain.

Clone: 1B6

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References

Meylan, Curran, Hofmann, Moradpour, Binder, Bartenschlager, Tschopp: "Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus." in: Nature, Vol. 437, Issue 7062, pp. 1167-72, (2005) (PubMed).

Kapadia, Chisari: "Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2561-6, (2005) (PubMed).

Moradpour, Evans, Gosert, Yuan, Blum, Goff, Lindenbach, Rice: "Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes." in: Journal of virology, Vol. 78, Issue 14, pp. 7400-9, (2004) (PubMed).

Gosert, Egger, Lohmann, Bartenschlager, Blum, Bienz, Moradpour: "Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons." in: Journal of virology, Vol. 77, Issue 9, pp. 5487-92, (2003) (PubMed).

Dimitrova, Imbert, Kieny, Schuster: "Protein-protein interactions between hepatitis C virus nonstructural proteins." in: Journal of virology, Vol. 77, Issue 9, pp. 5401-14, (2003) (PubMed).

Wölk, Sansonno, Kräusslich, Dammacco, Rice, Blum, Moradpour: "Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines." in: Journal of virology, Vol. 74, Issue 5, pp. 2293-304, (2000) (PubMed).

Target Details

Target: NS3

Target Type: Viral Protein

Background: The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.