ANGPTL5 Protein (AA 1-388) (GST tag)

Catalog No. ABIN1345060

Product Details

Target: ANGPTL5 (Angiopoietin-Like 5 (ANGPTL5))

Protein Type: Recombinant

Protein Characteristics: AA 1-388

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This ANGPTL5 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: ANGPTL5 (Human) Recombinant Protein (P01)

Sequence: MMSPSQASLL FLNVCIFICG EAVQGNCVHH STDSSVVNIV EDGSNAKDES KSNDTVCKED CEESCDVKTK ITREEKHFMC RNLQNSIVSY TRSTKKLLRN MMDEQQASLD YLSNQVNELM NRVLLLTTEV FRKQLDPFPH RPVQSHGLDC TDIKDTIGSV TKTPSGLYII HPEGSSYPFE VMCDMDYRGG GWTVIQKRID GIIDFQRLWC DYLDGFGDLL GEFWLGLKKI FYIVNQKNTS FMLYVALESE DDTLAYASYD NFWLEDETRF FKMHLGRYSG NAGDAFRGLK REDNQNAKPF STSDVDNDGC RPACLVNGQS VKSCSHLHNK TGWWFNECGL ANLNGIHHFS GKLLATGIQW GTWTKNNSPV KIKSVSMKIR RMYNPYFK

Characteristics: Human ANGPTL5 full-length ORF ( AAH49170.1, 1 a.a. - 388 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Drake, Khoury, Leskov, Iliopoulou, Fragoso, Lodish, Chen: "Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2r?-/- (NSG) mice." in: PLoS ONE, Vol. 6, Issue 4, pp. e18382, (2011) (PubMed).

Walenda, Bokermann, Ventura Ferreira, Piroth, Hieronymus, Neuss, Zenke, Ho, Müller, Wagner: "Synergistic effects of growth factors and mesenchymal stromal cells for expansion of hematopoietic stem and progenitor cells." in: Experimental hematology, Vol. 39, Issue 6, pp. 617-28, (2011) (PubMed).

Zhang, Kaba, Iizuka, Huynh, Lodish: "Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation." in: Blood, Vol. 111, Issue 7, pp. 3415-23, (2008) (PubMed).

Zhang, Kaba, Ge, Xie, Tong, Hug, Lodish: "Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells." in: Nature medicine, Vol. 12, Issue 2, pp. 240-5, (2006) (PubMed).

Target Details

Target: ANGPTL5 (Angiopoietin-Like 5 (ANGPTL5))

Alternative Name: ANGPTL5 (ANGPTL5 Products)

Synonyms: AGF Protein, ARP5 Protein, ANGPTL5 Protein, bZ1P14.8 Protein, wu:fe36e01 Protein, si:rp71-1p14.8 Protein, angiopoietin like 5 Protein, angiopoietin like 6 Protein, angiopoietin-like 5 Protein, ANGPTL5 Protein, ANGPTL6 Protein, angptl5 Protein, Angptl5 Protein

Background: Full Gene Name: angiopoietin-like 5
Synonyms:

Gene ID: 253935