JMY antibody (N-Term)

Catalog No. ABIN1881469

Product Details anti-JMY Antibody

Target: JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))

Binding Specificity: AA 2-29, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMY antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This JMY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-29 amino acids from the N-terminal region of human JMY.

Clone: RB30560

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-JMY antibody (ABIN1881469)

Coutts, Weston, La Thangue: "A transcription co-factor integrates cell adhesion and motility with the p53 response." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 47, pp. 19872-7, (2009) (PubMed).

Zuchero, Coutts, Quinlan, Thangue, Mullins: "p53-cofactor JMY is a multifunctional actin nucleation factor." in: Nature cell biology, Vol. 11, Issue 4, pp. 451-9, (2009) (PubMed).

Nordgard, Johansen, Alnaes, Bucher, Syvänen, Naume, Børresen-Dale, Kristensen: "Genome-wide analysis identifies 16q deletion associated with survival, molecular subtypes, mRNA expression, and germline haplotypes in breast cancer patients." in: Genes, chromosomes & cancer, Vol. 47, Issue 8, pp. 680-96, (2008) (PubMed).

Coutts, Boulahbel, Graham, La Thangue: "Mdm2 targets the p53 transcription cofactor JMY for degradation." in: EMBO reports, Vol. 8, Issue 1, pp. 84-90, (2007) (PubMed).

Shikama, Lee, France, Delavaine, Lyon, Krstic-Demonacos, La Thangue: "A novel cofactor for p300 that regulates the p53 response." in: Molecular cell, Vol. 4, Issue 3, pp. 365-76, (1999) (PubMed).

Target Details for JMY

Target: JMY (Junction Mediating and Regulatory Protein, P53 Cofactor (JMY))

Alternative Name: JMY (JMY Products)

Synonyms: WHDC1L3 antibody, junction mediating and regulatory protein, p53 cofactor antibody, junction-mediating and regulatory protein antibody, JMY antibody, jmy antibody, Jmy antibody

Background: JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).

Molecular Weight: 111445

NCBI Accession: NP_689618

UniProt: Q8N9B5

Pathways: Regulation of Actin Filament Polymerization