CYP1A2 Protein (AA 211-310) (GST tag)

Catalog No. ABIN1350938

Product Details

Target: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Protein Type: Recombinant

Protein Characteristics: AA 211-310

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This CYP1A2 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: CYP1A2 (Human) Recombinant Protein (Q01)

Sequence: ESSDEMLSLV KNTHEFVETA SSGNPLDFFP ILRYLPNPAL QRFKAFNQRF LWFLQKTVQE HYQDFDKNSV RDITGALFKH SKKGPRASGN LIPQEKIVNL

Characteristics: Human CYP1A2 partial ORF ( NP_000752, 211 a.a. - 310 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Kawakami, Ohtsuki, Kamiie, Suzuki, Abe, Terasaki: "Simultaneous absolute quantification of 11 cytochrome P450 isoforms in human liver microsomes by liquid chromatography tandem mass spectrometry with in silico target peptide selection." in: Journal of pharmaceutical sciences, Vol. 100, Issue 1, pp. 341-52, (2010) (PubMed).

Target Details

Target: CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))

Alternative Name: CYP1A2 (CYP1A2 Products)

Synonyms: CP12 Protein, P3-450 Protein, P450(PA) Protein, CYP1A2 Protein, CYPIA2 Protein, P450-PM4 Protein, Cyp1a1 Protein, P450-3 Protein, CYPD45 Protein, P-450d Protein, RATCYPD45 Protein, CYP1A5 Protein, CP450 Protein, CYP-IA2 Protein, cytochrome P450 family 1 subfamily A member 2 Protein, cytochrome P450 1A2 Protein, cytochrome P450 LM4 Protein, cytochrome P450, family 1, subfamily A, polypeptide 2 Protein, cytochrome P450, family 1, subfamily a, polypeptide 2 Protein, cytochrome P450 1A2-like Protein, CYP1A2 Protein, LOC100328937 Protein, Cyp1a2 Protein, VDBG_06455 Protein, LOC101825565 Protein, LOC102130662 Protein

Background: Full Gene Name: cytochrome P450, family 1, subfamily A, polypeptide 2
Synonyms: CP12,P3-450,P450(PA)

Gene ID: 1544

NCBI Accession: NM_000761