Nuclear Factor kappa B p50 (NFkB p50) (N-Term) antibody

Catalog No. ABIN233795

Product Details

Target: Nuclear Factor kappa B p50 (NFkB p50)

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins. Control peptide (code #100-4164p) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1).

Characteristics: Anti NFkB p50 Antibody recognizes NFKB p50 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.

Purification: affinity purified

Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide

Application Details

Application Notes: This product was assayed by immunoblot and found to be reactive against Human NFkB p50 (NFKB1) at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) Anti- Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105. No reaction was observed against the analogous mouse protein. This product was also tested in a gel supershift assay and found to be reactive against p50:p50 homodimers and p:50:p65 heterodimers using 0.5 to 1.0 µl per assay.

Comment:

Gene Name: NFKB1

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References

Hariri, Arguello, Volpon, Culjkovic-Kraljacic, Nielsen, Hiscott, Mann, Borden: "The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia." in: Leukemia, Vol. 27, Issue 10, pp. 2047-55, (2013) (PubMed).

Ghosh, Yang, Rothstein, Robinson: "Nuclear factor-κB contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 25, pp. 9159-69, (2011) (PubMed).

Target Details

Target: Nuclear Factor kappa B p50 (NFkB p50)

Alternative Name: NFKB p50

Synonyms: NF-KB1 antibody, NF-kappaB antibody, NF-kappaB1 antibody, p105 antibody, p50 antibody, p50/p105 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, Nfkb1 antibody

Background: Anti NFkB p50 Antibody recognizes NFKB p50 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB

Gene ID: 8792

UniProt: Q9Y6Q6