SensoLyte® FDP Protein Phosphatase Assay Kit

Catalog No. ABIN1882460

Product Details

Target: Fluorescein Diphosphate (FDP)

Detection Method: Fluorometric

Application: Enzyme Activity Assay (EAA)

Brand: SensoLyte®

Application Details

Comment:

Enzymatic Activity Assay kit

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Store all kit components at -20 °C. Components B, C, D, E and G can be stored at room temperature for convenience.

References

Bica, Liddell, Donnelly, Duncan, Caragounis, Volitakis, Paterson, Cappai, Grubman, Camakaris, Crouch, White: "Neuroprotective copper bis(thiosemicarbazonato) complexes promote neurite elongation." in: PLoS ONE, Vol. 9, Issue 2, pp. e90070, (2014) (PubMed).

Xie, Shi, Xie, Wu, Li, Hua, Bian: "Sulfhydration of p66Shc at cysteine59 mediates the antioxidant effect of hydrogen sulfide." in: Antioxidants & redox signaling, Vol. 21, Issue 18, pp. 2531-42, (2014) (PubMed).

Savinov, Pan, Ghosh, Hatfull: "The Bxb1 gp47 recombination directionality factor is required not only for prophage excision, but also for phage DNA replication." in: Gene, Vol. 495, Issue 1, pp. 42-8, (2012) (PubMed).

Zhang, Neganova, Przyborski, Yang, Cooke, Atkinson, Anyfantis, Fenyk, Keith, Hoare, Hughes, Strachan, Stojkovic, Hinds, Armstrong, Lako: "A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A." in: The Journal of cell biology, Vol. 184, Issue 1, pp. 67-82, (2009) (PubMed).

Kirchberger, Majdic, Blüml, Schrauf, Leitner, Gerner, Paster, Gundacker, Sibilia, Stöckl: "The cytoplasmic tail of CD45 is released from activated phagocytes and can act as an inhibitory messenger for T cells." in: Blood, Vol. 112, Issue 4, pp. 1240-8, (2008) (PubMed).

Target Details

Target: Fluorescein Diphosphate (FDP)

Alternative Name: FDP (FDP Products)

Background: Protein phosphatases have received great attention as drug-screening targets. FDP is a highly sensitive fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases. The phosphatase-induced hydrolysis of FDP yields an intense fluorescent product that has excitation wavelength around 490 nm, and emission maximum around 520 nm. The fluorescence of FDP hydrolyzed product is strengthened when pH > 7.0. For some protein phosphatase requiring slightly acidic buffer for reaction, a pH-adjusting stop solution should be added before reading the fluorescence signal. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inducers and inhibitors.