ATP2A2 Protein (AA 1-997) (GST tag)
(1 reference)-
- Target See all ATP2A2 Proteins
- ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-997
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This ATP2A2 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- ATP2A2 (Human) Recombinant Protein (P01)
- Sequence
- MENAHTKTVE EVLGHFGVNE STGLSLEQVK KLKERWGSNE LPAEEGKTLL ELVIEQFEDL LVRILLLAAC ISFVLAWFEE GEETITAFVE PFVILLILVA NAIVGVWQER NAENAIEALK EYEPEMGKVY RQDRKSVQRI KAKDIVPGDI VEIAVGDKVP ADIRLTSIKS TTLRVDQSIL TGESVSVIKH TDPVPDPRAV NQDKKNMLFS GTNIAAGKAM GVVVATGVNT EIGKIRDEMV ATEQERTPLQ QKLDEFGEQL SKVISLICIA VWIINIGHFN DPVHGGSWIR GAIYYFKIAV ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS DKTGTLTTNQ MSVCRMFILD RVEGDTCSLN EFTITGSTYA PIGEVHKDDK PVNCHQYDGL VELATICALC NDSALDYNEA KGVYEKVGEA TETALTCLVE KMNVFDTELK GLSKIERANA CNSVIKQLMK KEFTLEFSRD RKSMSVYCTP NKPSRTSMSK MFVKGAPEGV IDRCTHIRVG STKVPMTSGV KQKIMSVIRE WGSGSDTLRC LALATHDNPL RREEMHLEDS ANFIKYETNL TFVGCVGMLD PPRIEVASSV KLCRQAGIRV IMITGDNKGT AVAICRRIGI FGQDEDVTSK AFTGREFDEL NPSAQRDACL NARCFARVEP SHKSKIVEFL QSFDEITAMT GDGVNDAPAL KKAEIGIAMG SGTAVAKTAS EMVLADDNFS TIVAAVEEGR AIYNNMKQFI RYLISSNVGE VVCIFLTAAL GFPEALIPVQ LLWVNLVTDG LPATALGFNP PDLDIMNKPP RNPKEPLISG WLFFRYLAIG CYVGAATVGA AAWWFIAADG GPRVSFYQLS HFLQCKEDNP DFEGVDCAIF ESPYPMTMAL SVLVTIEMCN ALNSLSENQS LLRMPPWENI WLVGSICLSM SLHFLILYVE PLPLIFQITP LNVTQWLMVL KISLPVILMD ETLKFVARNY LEPAILE
- Characteristics
- Human ATP2A2 full-length ORF ( NP_001672.1, 1 a.a. - 997 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 111-253) protein (His tag)
Crystallography grade ATP2A2 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 111-253) protein (His tag)Crystallography grade ATP2A2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 314-756) protein (His tag)Crystallography grade ATP2A2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 314-756) protein (His tag)Crystallography grade ATP2A2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 1-1042) protein (Strep Tag)Crystallography grade ATP2A2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 1-1044) protein (Strep Tag)Crystallography grade ATP2A2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) (AA 314-756) protein (His tag)ATP2A2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Regulation of Sarcoplasmic Reticulum Ca2+ ATPase 2 (SERCA2) Activity by Phosphodiesterase 3A (PDE3A) in Human Myocardium: PHOSPHORYLATION-DEPENDENT INTERACTION OF PDE3A1 WITH SERCA2." in: The Journal of biological chemistry, Vol. 290, Issue 11, pp. 6763-76, (2015) (PubMed).
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: "Regulation of Sarcoplasmic Reticulum Ca2+ ATPase 2 (SERCA2) Activity by Phosphodiesterase 3A (PDE3A) in Human Myocardium: PHOSPHORYLATION-DEPENDENT INTERACTION OF PDE3A1 WITH SERCA2." in: The Journal of biological chemistry, Vol. 290, Issue 11, pp. 6763-76, (2015) (PubMed).
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- Target
- ATP2A2 (ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2))
- Alternative Name
- ATP2A2 (ATP2A2 Products)
- Synonyms
- atp2b Protein, ca-p60a Protein, dar Protein, serca2 Protein, ATP2A2 Protein, ATP2B Protein, DAR Protein, DD Protein, SERCA2 Protein, SERCA2A Protein, ATP2 Protein, Serca2 Protein, SercaII Protein, 9530097L16Rik Protein, D5Wsu150e Protein, SERCA2B Protein, mKIAA4195 Protein, atp2a2 Protein, zgc:55380 Protein, ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 S homeolog Protein, ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 Protein, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 Protein, ATPase, Ca++ transporting, cardiac muscle, slow twitch 2a Protein, atp2a1.S Protein, ATP2A2 Protein, Atp2a2 Protein, atp2a2a Protein
- Background
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Full Gene Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms: ATP2B,DAR,DD,DKFZp686P0211,FLJ20293,FLJ38063,MGC45367,SERCA2 - Gene ID
- 488
- NCBI Accession
- NM_001681
- Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process
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