BLA-DQB antibody

Catalog No. ABIN2191836

Product Details anti-BLA-DQB Antibody

Target: BLA-DQB (MHC Class II Antigen (BLA-DQB))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Sterility: 0.2 μm filtered

Clone: ER-TR3

Application Details

Application Notes: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-BLA-DQB antibody (ABIN2191836)

Van Vliet, Jenkinson, Kingston, Owen, Van Ewijk: "Stromal cell types in the developing thymus of the normal and nude mouse embryo." in: European journal of immunology, Vol. 15, Issue 7, pp. 675-81, (1985) (PubMed).

Target Details for BLA-DQB

Target: BLA-DQB (MHC Class II Antigen (BLA-DQB))

Alternative Name: Major Histocompatibility Complex Class II (BLA-DQB Products)

Synonyms: BoLA-DQB antibody, DQB antibody, DQB1 antibody, OLA-DQB antibody, MHC class II antigen antibody, BLA-DQB antibody

Background: The monoclonal antibody ER-TR3 reacts with mouse major histocompatibitity complex class II (MHC class II), also known as I-A or I-E. MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains. Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes. First, the antigens endocytosed by the antigen-presenting cells must be broken down into antigenic peptides. Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they acquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside. MHC class II molecules present exogenously derived antigen to + CD4 T lymphocytes, which are usually T helper cells. CD4 interacts with non-polymorphic residues of MHC Class II. The monoclonal antibody ER-TR3 is a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells and macrophages) and for studying the development of T helper cells since they stain stromal cells in thymus. The level of antigen detected by ER-TR3 differs from strain to strain. The monoclonal antibody ER-TR3 cross reacts with human MHC class II on tonsil. Distribution of ER-TR3 among mouse strains with independent and recombinant haplotypes. Strain Haplotype