ZONA OCCLUDENS 1 alpha-Plus antibody
Reactivity: Human
WB, FACS, IA, IHC (fro), IHC (p)
Host: Rabbit
Polyclonal
unconjugated
(5 references)-
- Target
- ZONA OCCLUDENS 1 alpha-Plus
- Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Sterility
- 0.2 μm filtered
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- Application Notes
- For immunohistology, immunfluorescence, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50..
- Restrictions
- For Research Use only
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- Buffer
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- Expiry Date
- 12 months
-
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: "Differential distribution of the tight-junction-associated protein ZO-1 isoforms alpha+ and alpha- in guinea pig Sertoli cells: a possible association with F-actin and G-actin." in: Biology of reproduction, Vol. 57, Issue 2, pp. 367-76, (1997) (PubMed).
: "Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells." in: Journal of cell science, Vol. 108 ( Pt 4), pp. 1735-42, (1995) (PubMed).
: "Two classes of tight junctions are revealed by ZO-1 isoforms." in: The American journal of physiology, Vol. 264, Issue 4 Pt 1, pp. C918-24, (1993) (PubMed).
: "Localization and differential expression of two isoforms of the tight junction protein ZO-1." in: The American journal of physiology, Vol. 262, Issue 5 Pt 1, pp. C1119-24, (1992) (PubMed).
: "Diversity among tight junctions in rat kidney: glomerular slit diaphragms and endothelial junctions express only one isoform of the tight junction protein ZO-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 15, pp. 7075-9, (1992) (PubMed).
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: "Differential distribution of the tight-junction-associated protein ZO-1 isoforms alpha+ and alpha- in guinea pig Sertoli cells: a possible association with F-actin and G-actin." in: Biology of reproduction, Vol. 57, Issue 2, pp. 367-76, (1997) (PubMed).
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- Target
- ZONA OCCLUDENS 1 alpha-Plus
- Background
- The polyclonal antibody recognizes human zona occludens 1 (ZO-1), an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain- containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and
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