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Mouse (Murine) Angiopoietin 2 ELISA Kit

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Antigen
Angiopoietin 2 (ANGPT2) ELISA Kits
  • AGPT2
  • Agpt2
  • ANG-2
  • Ang-2
  • ANG2
  • ang2
  • Ang2
  • ANGIOPOIETIN-2
  • ANGPT2
  • angpt2
Epitope
AA 1-496
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
25
14
10
10
8
5
4
3
3
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672851
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32670403 ABIN415586 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32670403 ABIN1113420 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32670403 ABIN2708419 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
0.001210097 ABIN365441 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate 0.156-10 pg/mL 0.156 pg/mL Log in to see
0.001210097 ABIN627489 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 5-100 ng/mL 5 ng/mL Log in to see
0.001210097 ABIN1568362 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.001210097 ABIN511908 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.001210097 ABIN956375 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001210097 ABIN2042347 Sandwich ELISA 6-10000 pg/mL 6 pg/mL Log in to see
0.001210097 ABIN2683446 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.001210097 ABIN454258 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.001210097 ABIN2541294 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.001210097 ABIN2945236 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001210097 ABIN2055914 Sandwich ELISA Log in to see

Top referenced Angiopoietin 2 ELISA Kit for ELISA

Similar Angiopoietin 2 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 15 ELISA Kits

General

Antigen Angiopoietin 2 (ANGPT2) ELISA Kits
Epitope AA 1-496
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(25), (14), (10), (10), (8), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details Angiopoietin 2 ELISA Kit

Target details Application Details Handling References for Angiopoietin 2 Kit (ABIN1672851) Images
Purpose For quantitative detection of mouse Angiopoietin-2 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse Angiopoietin-2
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse Angiopoietin-2 antibod - 1
  • Lyophilized recombinant mouse Angiopoietin-2 standar - 10ng/tubex2
  • Biotinylated anti- mouse Angiopoietin-2 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: CHO
Immunogen sequence: M1-F496

Target details

Product Details Angiopoietin 2 ELISA Kit Application Details Handling References for Angiopoietin 2 Kit (ABIN1672851) Images back to top
Antigen
Alternative Name ANGPT2 (ANGPT2 ELISA Kit Abstract)
Background Angiopoietin-2 is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. Human and mouse ANGPT2 are 85 % identical in amino acid sequence and approximately 60 % identical to their ANGPT1 homologs. Transgenic overexpression of ANGPT2 in mice disrupted blood vessel formation in the mouse embryo. By FISH and radiation hybrid analysis, the human ANGPT2 gene was mapped to 8p23, and in a region of syntenic homology on mouse chromosome 8, where the Angpt2 gene was mapped. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis .
Gene ID 11601
UniProt B9EHQ4
Pathways RTK Signaling

Application Details

Product Details Angiopoietin 2 ELISA Kit Target details Handling References for Angiopoietin 2 Kit (ABIN1672851) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse Angiopoietin-2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(CHO, M1-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Angiopoietin-2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Angiopoietin-2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.15, Standard deviation: 0.044, CV(%): 3.8
  • Sample 2: n=16, Mean(ng/ml): 3.53, Standard deviation: 0.148, CV(%): 4.2
  • Sample 3: n=16, Mean(ng/ml): 7.21, Standard deviation: 0.368, CV(%): 5.1,
  • Sample 1: n=24, Mean(ng/ml): 1.18, Standard deviation: 0.103, CV(%): 8.7
  • Sample 2: n=24, Mean(ng/ml): 3.62, Standard deviation: 0.308, CV(%): 8.5
  • Sample 3: n=24, Mean(ng/ml): 7.34, Standard deviation: 0.558, CV(%): 7.6
Restrictions For Research Use only

Handling

Product Details Angiopoietin 2 ELISA Kit Target details Application Details References for Angiopoietin 2 Kit (ABIN1672851) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Angiopoietin 2 Kit (ABIN1672851)

Product Details Angiopoietin 2 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Kim, Moon, Koh, Kim, Uhm, Kwak, Kim, Koh: "Molecular cloning, expression, and characterization of angiopoietin-related protein. angiopoietin-related protein induces endothelial cell sprouting." in: The Journal of biological chemistry, Vol. 274, Issue 37, pp. 26523-8, 1999

Cheung, Stewart, Marsden: "Endothelial Tie2/Tek ligands angiopoietin-1 (ANGPT1) and angiopoietin-2 (ANGPT2): regional localization of the human genes to 8q22.3-q23 and 8p23." in: Genomics, Vol. 48, Issue 3, pp. 389-91, 1998

Images

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Supplier Images
ELISA image for Angiopoietin 2 ELISA Kit (ANGPT2) (ABIN1672851) Mouse Angiopoietin-2 PicoKine ELISA Kit standard curve