anti-Human Betacellulin antibody for Immunofluorescence

Recommended Betacellulin Antibody (supplied by: Log in to see )

Antigen
Betacellulin (BTC) Antibodies
  • BTC
  • LOC100228121
  • probetacellulin
  • Bcn
  • betacellulin
  • betacellulin-like
  • betacellulin, epidermal growth factor family member
  • BTC
  • LOC100228121
  • Btc
  • LOC100343154
Epitope
AA 17-45, N-Term
11
7
7
7
5
5
3
2
1
Reactivity
Human
96
29
5
3
3
Host
Rabbit
77
24
16
1
1
Clonality
Polyclonal
Conjugate
This Betacellulin antibody is un-conjugated
14
5
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
97
37
34
23
13
7
7
6
3
3
2
2
2
1
1
Options
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Catalog No. ABIN655615
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.326117 ABIN4284565 FACS ICC IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN5556196 EIA FACS IF IHC (p) WB Rabbit Ig Fraction AA 24-54, N-Term Log in to see Polyclonal
1 ABIN5530625 FACS IF IHC (p) WB Rabbit Ig Fraction AA 17-45, N-Term Log in to see Polyclonal
1 ABIN2210123 FACS IF IHC ELISA WB Rabbit IgG N-Term, AA 24-54 Log in to see Polyclonal
1 ABIN1806213 FACS IF IHC (p) WB Rabbit AA 17-45 Log in to see Polyclonal
1 ABIN2797423 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal

Top referenced anti-Betacellulin antibody for Immunofluorescence

Similar anti-Betacellulin Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
ELISA 32 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 8 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Neutralization (Neut) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 76 Antibodies

General

Antigen Betacellulin (BTC) Antibodies
Epitope AA 17-45, N-Term
(11), (7), (7), (7), (5), (5), (3), (2), (1)
Reactivity Human
(96), (29), (5), (3), (3)
Host Rabbit
(77), (24), (16), (1), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Betacellulin antibody is un-conjugated
(14), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(97), (37), (34), (23), (13), (7), (7), (6), (3), (3), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-Betacellulin Antibody

Target Details Betacellulin Application Details Handling ProductDetails: References for anti-Betacellulin antibody (ABIN655615) Images
Specificity This BTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human BTC.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This BTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 AA from the N-terminal region of human BTC.
Clone RB14431
Isotype Ig

Target Details Betacellulin

Product Details anti-Betacellulin Antibody Application Details Handling ProductDetails: References for anti-Betacellulin antibody (ABIN655615) Images back to top
Antigen
Alternative Name BTC (BTC Antibody Abstract)
Background The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.
Synonyms: RB14431
Pathways: ErbB signaling pathway
Molecular Weight 19746 Da
Gene ID 685
UniProt P35070
Research Area Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-Betacellulin Antibody Target Details Betacellulin Handling ProductDetails: References for anti-Betacellulin antibody (ABIN655615) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Betacellulin Antibody Target Details Betacellulin Application Details ProductDetails: References for anti-Betacellulin antibody (ABIN655615) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-Betacellulin antibody (ABIN655615)

Product Details anti-Betacellulin Antibody Target Details Betacellulin Application Details Handling Images back to top
Background publications

Stoeck, Shang, Dempsey: "Sequential and gamma-secretase-dependent processing of the betacellulin precursor generates a palmitoylated intracellular-domain fragment that inhibits cell growth." in: Journal of cell science, Vol. 123, Issue Pt 13, pp. 2319-31, 2010

Genetos, Rao, Vidal: "Betacellulin inhibits osteogenic differentiation and stimulates proliferation through HIF-1alpha." in: Cell and tissue research, Vol. 340, Issue 1, pp. 81-9, 2010

Nagaoka, Fukuda, Hashizume, Nishiyama, Tada, Yamada, Salomon, Yamada, Kojima, Seno: "A betacellulin mutant promotes differentiation of pancreatic acinar AR42J cells into insulin-producing cells with low affinity of binding to ErbB1." in: Journal of molecular biology, Vol. 380, Issue 1, pp. 83-94, 2008

Moss, Bomar, Liu, Sage, Dempsey, Lenhart, Gillispie, Stoeck, Wildeboer, Bartsch, Palmisano, Zhou: "The ADAM10 prodomain is a specific inhibitor of ADAM10 proteolytic activity and inhibits cellular shedding events." in: The Journal of biological chemistry, Vol. 282, Issue 49, pp. 35712-21, 2007

Révillion, Lhotellier, Hornez, Bonneterre, Peyrat: "ErbB/HER ligands in human breast cancer, and relationships with their receptors, the bio-pathological features and prognosis." in: Annals of oncology : official journal of the European Society for Medical Oncology / ESMO, Vol. 19, Issue 1, pp. 73-80, 2007

Images

Product Details anti-Betacellulin Antibody Target Details Betacellulin Application Details Handling ProductDetails: References for anti-Betacellulin antibody (ABIN655615) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Betacellulin antibody (BTC) (AA 17-45) (ABIN655615) Confocal immunofluorescent analysis of BTC Antibody (N-term)(ABIN655615) with MDA-MB2...