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Human Betacellulin ELISA Kit

Recommended Betacellulin Product (supplied by: Log in to see )

Antigen
Betacellulin (BTC) ELISA Kits
  • Bcn
  • BTC
  • LOC100228121
  • probetacellulin
Epitope
AA 32-111
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
13
9
4
3
3
3
3
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN1672881
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414356 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.32693866 ABIN924755 Sandwich ELISA Cell Lysate, Serum, Plasma 8-1000 pg/mL 8 pg/mL Log in to see
0.32693866 ABIN1113754 Sandwich ELISA Serum, Plasma, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN924892 Log in to see
0.32693866 ABIN1028203 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32693866 ABIN2708080 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN828615 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN3032899 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 24.7-18000 pg/mL 24.7 pg/mL Log in to see
0.0012117105 ABIN511243 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012117105 ABIN625268 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.0012117105 ABIN2042359 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2113186 Cell Lysate, Serum, Plasma 8-1000 pg/mL 8 pg/mL Log in to see
0.0012117105 ABIN2683570 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
0.0012117105 ABIN2542051 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN454519 78-5000 pg/mL 78 pg/mL Log in to see
0.0012117105 ABIN2067316 Sandwich ELISA Log in to see
0.0012117105 ABIN2945673 15.625-1000 pg/mL 15.625 pg/mL Log in to see

Top referenced Betacellulin ELISA Kit for ELISA

Similar Betacellulin ELISA Kits

Application / Reactivity Human
ELISA 18 ELISA Kits

General

Antigen Betacellulin (BTC) ELISA Kits
Epitope AA 32-111
Reactivity Human
Kits with alternative reactivity to:
(17), (13), (9), (4), (3), (3), (3), (3), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 3 references available
Supplier Log in to see

Product Details Betacellulin ELISA Kit

Target details Application Details Handling References for Betacellulin Kit (ABIN1672881) Images
Purpose For quantitative detection of human Betacellulin in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human Betacellulin
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human Betacellulin antibod - 1
  • Lyophilized recombinant human Betacellulin standar - 10ng/tubex2
  • Biotinylated anti- human Betacellulin antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: D32-Y111

Target details

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Antigen
Alternative Name BTC (BTC ELISA Kit Abstract)
Background Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Gene ID 685
UniProt P35070
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Betacellulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Betacellulin has been precoated onto 96-well plates. Standards(E.coli, D32-Y111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Betacellulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Betacellulin amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human Betacellulin standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Betacellulin standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 46, Standard deviation: 2.35, CV(%): 5.1
  • Sample 2: n=16, Mean(pg/ml): 119, Standard deviation: 6.31, CV(%): 5.3
  • Sample 3: n=16, Mean(pg/ml): 725, Standard deviation: 45.68, CV(%): 6.3,
  • Sample 1: n=24, Mean(pg/ml): 47, Standard deviation: 2.73, CV(%): 5.8
  • Sample 2: n=24, Mean(pg/ml): 124, Standard deviation: 7.69, CV(%): 6.2
  • Sample 3: n=24, Mean(pg/ml): 736, Standard deviation: 58.14, CV(%): 7.9
Restrictions For Research Use only

Handling

Product Details Betacellulin ELISA Kit Target details Application Details References for Betacellulin Kit (ABIN1672881) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Betacellulin Kit (ABIN1672881)

Product Details Betacellulin ELISA Kit Target details Application Details Handling Images back to top
Background publications

Park, Su, Ariga, Law, Jin, Conti: "EGF-like growth factors as mediators of LH action in the ovulatory follicle." in: Science (New York, N.Y.), Vol. 303, Issue 5658, pp. 682-4, 2004

Watanabe, Shintani, Nakata, Shing, Folkman, Igarashi, Sasada: "Recombinant human betacellulin. Molecular structure, biological activities, and receptor interaction." in: The Journal of biological chemistry, Vol. 269, Issue 13, pp. 9966-73, 1994

Sasada, Ono, Taniyama, Shing, Folkman, Igarashi: "Cloning and expression of cDNA encoding human betacellulin, a new member of the EGF family." in: Biochemical and biophysical research communications, Vol. 190, Issue 3, pp. 1173-9, 1993

Images

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Supplier Images
ELISA image for Betacellulin ELISA Kit (BTC) (ABIN1672881) Human Betacellulin/BTC PicoKine ELISA Kit standard curve