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Human Betacellulin ELISA Kit

Recommended Betacellulin Product (supplied by: Log in to see )

Antigen
Betacellulin (BTC) ELISA Kits
  • BTC
  • LOC100228121
  • probetacellulin
  • Bcn
  • betacellulin
  • betacellulin-like
  • betacellulin, epidermal growth factor family member
  • BTC
  • LOC100228121
  • Btc
  • LOC100343154
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
18
14
10
4
4
2
2
2
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
30-18000 pg/mL
Minimum Detection Limit
30 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625268
$ 666.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.840559 ABIN1672881 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 15.6-1000 pg/mL 15.6 pg/mL Log in to see 3
10.840559 ABIN414356 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
10.840559 ABIN924755 Sandwich ELISA Cell Lysate, Serum, Plasma 8-1000 pg/mL 8 pg/mL Log in to see
10.840559 ABIN1113754 Sandwich ELISA Serum, Plasma, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
10.840559 ABIN1028203 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
10.840559 ABIN924892 Log in to see
10.840559 ABIN2708080 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN828615 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN3032899 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 24.7-18000 pg/mL 24.7 pg/mL Log in to see
1 ABIN511243 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN2113186 Cell Lysate, Serum, Plasma 8-1000 pg/mL 8 pg/mL Log in to see
1 ABIN2042359 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
1 ABIN2683570 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see
1 ABIN2542051 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN4967496 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN454519 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN2945673 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN5073586 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

Top referenced Betacellulin ELISA Kit for ELISA

  • Human Betacellulin ELISA Kit for Sandwich ELISA - ABIN625268 (1 Publications): Lim, Yoo, Kim, Hur, Lee, Hur, Lee, Lee, Park, Lee, Chang, Kim, Kang, Hong, Kim, Kim, Yoon, Nam, Yang, Kim, Cho, Won: GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands. in Molecular cancer therapeutics 2016 (PubMed)

Similar Betacellulin ELISA Kits

Application / Reactivity Human
ELISA 19 ELISA Kits

General

Antigen Betacellulin (BTC) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(18), (14), (10), (4), (4), (2), (2), (2), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 30-18000 pg/mL
Minimum Detection Limit 30 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details Betacellulin ELISA Kit

Target details Application Details Handling References for Betacellulin Kit (ABIN625268) Images
Purpose Human Betacellulin (BTC) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity < 30 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details Betacellulin ELISA Kit Application Details Handling References for Betacellulin Kit (ABIN625268) Images back to top
Antigen
Background The Human BTC (Betacellulin) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BTC in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human BTC coated on a 96-well plate. Standards and samples are pipetted into the wells and BTC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BTC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BTC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 685
UniProt P35070
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details Betacellulin ELISA Kit Target details Handling References for Betacellulin Kit (ABIN625268) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
2. Sample dilution: If your samples need to be diluted, 1x Assay Diluent D (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernantants and urine. Suggested dilution for normal serum/plasma: 2 fold*. *Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
3. Assay Diluent D (Item K) and Assay Diluent B (Item E) should be diluted 5-fold with deionized or distilled water before use.
4. Preparation of standard: Briefly spin the vial of Item C. Add 400 µL 1x Assay Diluent D (Item K) into Item C vial to prepare a 50 ng/mL standard solution. Dissolve the powder thoroughly by a gentle mix. Add 180 µL BTC standard from the vial of Item C, into a tube with 320 µL 1x Assay Diluent D to prepare a 18,000 pg/mL standard solution. Pipette 400myl 1x Assay Diluent D into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. 1x Assay Diluent D serves as the zero standard (0 pg/mL). 200 µL 200 µL 200 µL 200 µL 200 µL 200myl 180 µL standard + 320 µL 18,000 6,000 2,000 666.7 222.2 74.07 24.69 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B (Item E) into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent Band used in step 4 of Part VI Assay Procedure.
7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 240-fold with 1x Assay Diluent B (Item E). For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 12 ml 1x Assay Diluent B to prepare a final 240 fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Discard the solution. Repeat the wash as in step
6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
7. Discard the solution. Repeat the wash as in step
8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent D Human BTC concentration (pg/mL) O D =4 50 n m 0.1 1 10 10 100 1,000 10,000 100,000
Sensitivity: The minimum detectable dose of BTC is typically less than 30 pg/mL.
Recovery: Recovery was determined by spiking various levels of BTC into normal human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 96.23 87-105 Plasma 78.86 67-88 Cell culture media 74.34 65-85
Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 86.24 84.48 87.53 Range ( %) 73-94 72-92 77-96 1:4 Average % of Expected 78.34 75.39 74.78 Range ( %) 67-87 67-86 66-85
Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %
Assay Precision Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
Restrictions For Research Use only

Handling

Product Details Betacellulin ELISA Kit Target details Application Details References for Betacellulin Kit (ABIN625268) Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for Betacellulin Kit (ABIN625268)

Product Details Betacellulin ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Lim, Yoo, Kim, Hur, Lee, Hur, Lee, Lee, Park, Lee, Chang, Kim, Kang, Hong, Kim, Kim, Yoon, Nam, Yang, Kim, Cho, Won: "GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands." in: Molecular cancer therapeutics, Vol. 15, Issue 2, pp. 251-63, 2016

Images

Product Details Betacellulin ELISA Kit Target details Application Details Handling References for Betacellulin Kit (ABIN625268) back to top
Supplier Images
ELISA image for Betacellulin ELISA Kit (BTC) (ABIN625268) Betacellulin (BTC) ELISA Kit