Human EPH Receptor A7 ELISA Kit

Recommended EPH Receptor A7 Product (supplied by: Log in to see )

Antigen
EPH Receptor A7 (EPHA7) ELISA Kits
  • fc51g03
  • wu:fc51g03
  • si:dkey-23k6.1
  • EHK3
  • HEK11
  • Cek11
  • Ebk
  • Ehk3
  • Hek11
  • Mdk1
  • CEPHA7
  • EPH receptor A7
  • eph receptor A7
  • Eph receptor A7
  • EPHA7
  • epha7
  • LOC100224370
  • Epha7
Epitope
pTyr
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
4
3
3
Method Type
Sandwich ELISA
Application
ELISA
Options
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Catalog No. ABIN4886068
$ 728.20
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1381110 Cell ELISA Log in to see
1 ABIN2113795 Cell ELISA Log in to see
1 ABIN2684130 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see

General

Antigen EPH Receptor A7 (EPHA7) ELISA Kits
Epitope pTyr
Reactivity Human
Kits with alternative reactivity to:
(4), (3), (3)
Method Type Sandwich ELISA
Application ELISA
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Product Details EPH Receptor A7 ELISA Kit

Target details Application Details Handling Images
Purpose Custom Human Phosphotyrosine EphA7 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphA7 in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Detection Method Colorimetric
Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphA7.
Characteristics
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Biotinylated Anti-Phosphotyrosine Antibody
  • Stop Solution
  • Assay Diluent(s)
  • Positive Control Sample
  • Lysis Buffer
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details EPH Receptor A7 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name EphA7 (EPHA7 ELISA Kit Abstract)
Gene ID 2045
UniProt Q15375
Pathways RTK Signaling

Application Details

Product Details EPH Receptor A7 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details EPH Receptor A7 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months