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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||3 Antibodies||50 Antibodies|
|Flow Cytometry (FACS)||11 Antibodies||27 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies||37 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||27 Antibodies|
|Western Blotting (WB)||10 Antibodies||81 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Antigen||EPH Receptor B3 (EPHB3) Antibodies|
|Epitope||AA 982-998, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This EPH Receptor B3 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-EPH Receptor B3 AntibodyTarget Details EPH Receptor B3 Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Ephrin type-B receptor 3(EPHB3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: EPH receptor B3
Protein Name: Ephrin type-B receptor 3
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor B3(982-998aa SIQDMRLQMNQTLPVQV), identical to the related rat and mouse sequences.|
Target Details EPH Receptor B3Product Details anti-EPH Receptor B3 Antibody Application Details Handling Images back to top
|Alternative Name||EPHB3 (EPHB3 Antibody Abstract)|
|Background||Ephrin Receptor EphB3, is also known as human embryo kinase2(HEK2) or Eph-like tyrosine kinase2(ETK2). HEK2, which is a member of the EPH/ELK family of tyrosine kinases, encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and 2 fibronectin repeats. The EPHB3 gene is mapped to human chromosome 3q21-qter. HEK2 interacts with 2 ligands of EPH-related kinases(LERKs), namely, LERK2(EFNB1) and LERK5(EFNB2). Coincubation of HEK2- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of HEK2 and LERK2 results in reduced kinase activity of HEK2.|
Application DetailsProduct Details anti-EPH Receptor B3 Antibody Target Details EPH Receptor B3 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPH Receptor B3 Antibody Target Details EPH Receptor B3 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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