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anti-Human Galectin 3 antibody for Immunoassay

Recommended Galectin 3 Antibody (supplied by: Log in to see )

Antigen
Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
  • CBP 35
  • CBP35
  • gal-3
  • Gal-3
  • GAL3
  • gal3
  • galactoside-binding
  • GALBP
  • Galectin-3
  • galectin-3
  • GALIG
  • GBP
  • L-34
  • L31
  • lectin
  • leg3
  • LGALS2
  • LGALS3
  • Mac-2
  • MAC2
  • MGC52947
  • MGC75803
  • soluble
Reactivity
Human
358
178
90
14
4
3
3
2
Host
Mouse
214
177
51
14
Clonality
Monoclonal
Conjugate
This Galectin 3 antibody is un-conjugated
37
22
19
15
10
10
6
6
4
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
320
201
146
107
81
78
46
36
32
12
10
6
6
4
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN449145
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008700774 ABIN2192045 Func IA IHC (fro) FACS IHC (p) WB Mouse Log in to see B2C10 3
0.0008700774 ABIN1110956 CA DB IA ISt ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-Galectin 3 antibody for Immunoassay

Similar anti-Galectin 3 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 146 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Functional Studies (Func) 4 Antibodies
Immunoassay (IA) 3 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 47 Antibodies
Immunofluorescence (IF) 81 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 128 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 81 Antibodies
Immunoprecipitation (IP) 12 Antibodies

General

Antigen Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
Reactivity Human
(358), (178), (90), (14), (4), (3), (3), (2)
Host Mouse
(214), (177), (51), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Galectin 3 antibody is un-conjugated
(37), (22), (19), (15), (10), (10), (6), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunoassay (IA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(320), (201), (146), (107), (81), (78), (46), (36), (32), (12), (10), (6), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-Galectin 3 Antibody

Target Details Galectin 3 Application Details Handling Images
Specificity The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3's hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.
Purification IgG purified
Clone B2C10
Isotype IgG1

Target Details Galectin 3

Product Details anti-Galectin 3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Galectin-3 (LGALS3 Antibody Abstract)
Background Gene Symbol: LGALS3
Gene ID 3958
Research Area Neurology
Pathways RTK Signaling

Application Details

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Handling Images back to top
Application Notes The monoclonal antibody B2C10 can be used for Western blotting, flow cytometry and immunohistology on both frozen and paraffin embedded sections. Furthermore the monoclonal antibody B2C10 is useful for immuno assays and functional studies.For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of galectin-3 to be inhibited.
Recommended dilutions: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunoassay, Functional, Immunohistochemistry 1:10-1:500
Restrictions For Research Use only

Handling

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details Images back to top
Concentration 0.1 mg/mL
Buffer PBS, containing 0.1 % bovine serum albumin.
Preservative Without preservative
Handling Advice Do not freeze.
Storage 4 °C