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Mouse (Murine) Galectin 3 ELISA Kit

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Antigen
Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) ELISA Kits
  • CBP 35
  • CBP35
  • gal-3
  • Gal-3
  • GAL3
  • gal3
  • galactoside-binding
  • GALBP
  • Galectin-3
  • galectin-3
  • GALIG
  • GBP
  • L-34
  • L31
  • lectin
  • leg3
  • LGALS2
  • LGALS3
  • Mac-2
  • MAC2
  • MGC52947
  • MGC75803
  • soluble
Epitope
AA 2-264
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
26
20
15
7
5
4
4
3
3
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672793
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32670116 ABIN415692 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32670116 ABIN1115021 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32670116 ABIN1569961 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.32670116 ABIN2708512 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012101001 ABIN578370 Sandwich ELISA Serum, Plasma, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Log in to see 1
0.0012101001 ABIN3032804 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 8.2-2000 pg/mL 8.2 pg/mL Log in to see
0.0012101001 ABIN625256 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see 4
0.0012101001 ABIN627871 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
0.0012101001 ABIN2748119 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 3-800 pg/mL Log in to see
0.0012101001 ABIN512695 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012101001 ABIN1381157 Cell ELISA Log in to see
0.0012101001 ABIN2042478 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012101001 ABIN2684863 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012101001 ABIN2064725 Cell ELISA Log in to see
0.0012101001 ABIN579630 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012101001 ABIN2684864 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see
0.0012101001 ABIN2684862 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
0.0012101001 ABIN2533672 Sandwich ELISA Serum, Plasma, Biological Fluids 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.0012101001 ABIN2947661 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012101001 ABIN2083720 Sandwich ELISA Log in to see

Top referenced Galectin 3 ELISA Kit for ELISA

Similar Galectin 3 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 21 ELISA Kits

General

Antigen Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) ELISA Kits
Epitope AA 2-264
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(26), (20), (15), (7), (5), (4), (4), (3), (3), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 3 references available
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Product Details Galectin 3 ELISA Kit

Target details Application Details Handling References for Galectin 3 Kit (ABIN1672793) Images
Purpose For quantitative detection of mouse Galectin-3 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse Galectin-3
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse Galectin-3antibod - 1
  • Lyophilized recombinant mouse Galectin-3 standar - 10ng/tubex2
  • Biotinylated anti- mouse Galectin-3 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: A2-I264

Target details

Product Details Galectin 3 ELISA Kit Application Details Handling References for Galectin 3 Kit (ABIN1672793) Images back to top
Antigen
Alternative Name LGALS3 (LGALS3 ELISA Kit Abstract)
Background Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.
Gene ID 16854
UniProt Q8C253
Pathways RTK Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse Galectin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-3 has been precoated onto 96-well plates. Standards(E.coli, A2-I264) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-3 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Galectin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Galectin-3 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 850, Standard deviation: 37.4, CV(%): 4.4
  • Sample 2: n=16, Mean(pg/ml): 1347, Standard deviation: 66, CV(%): 4.9
  • Sample 3: n=16, Mean(pg/ml): 2932, Standard deviation: 161.3, CV(%): 5.5,
  • Sample 1: n=24, Mean(pg/ml): 795, Standard deviation: 54.06, CV(%): 6.8
  • Sample 2: n=24, Mean(pg/ml): 1046, Standard deviation: 99.83, CV(%): 7.1
  • Sample 3: n=24, Mean(pg/ml): 2758, Standard deviation: 201.3, CV(%): 7.3
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Galectin 3 Kit (ABIN1672793)

Product Details Galectin 3 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Dumic, Dabelic, Flögel: "Galectin-3: an open-ended story." in: Biochimica et biophysica acta, Vol. 1760, Issue 4, pp. 616-35, 2006

Liu, Patterson, Wang: "Intracellular functions of galectins." in: Biochimica et biophysica acta, Vol. 1572, Issue 2-3, pp. 263-73, 2002

Cooper: "Galectinomics: finding themes in complexity." in: Biochimica et biophysica acta, Vol. 1572, Issue 2-3, pp. 209-31, 2002

Images

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Supplier Images
ELISA image for Galectin 3 ELISA Kit (Lectin, Galactoside-Binding, Soluble, 3) (ABIN1672793) Mouse Galectin-3/LGALS3 PicoKine ELISA Kit standard curve