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Human HBEGF ELISA Kit

Recommended HBEGF Product (supplied by: Log in to see )

Antigen
Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA Kits
  • AW047313
  • Dtr
  • DTR
  • DTS
  • Dts
  • DTSF
  • GFHB
  • HB-EGF
  • Hb-egf
  • hbegf
  • HBEGF
  • HEGFL
  • Hegfl
  • zgc:165443
  • zgc:175091
Epitope
AA 63-148
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
19
13
13
3
3
3
3
2
2
2
2
1
Methode Type
Sandwich ELISA
Detection Range
15.6-1000 pg/mL
Minimum Detection Limit
15.6 pg/mL
Application
ELISA
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Catalog No. ABIN1672797
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414670 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.32693866 ABIN2708238 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN366089 Sandwich ELISA Serum, Plasma, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN3032930 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 16.38-4000 pg/mL 16.38 pg/mL Log in to see
0.0012117105 ABIN509985 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see
0.0012117105 ABIN625303 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 2
0.0012117105 ABIN455334 Serum, Tissue Homogenate, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN1028812 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012117105 ABIN770829 Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
0.0012117105 ABIN2684422 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine Log in to see 1
0.0012117105 ABIN2042490 Sandwich ELISA 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2684421 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 15.6-1000 pg/mL 15.6 pg/mL Log in to see
0.0012117105 ABIN2534391 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012117105 ABIN2534224 Tissue Homogenate, Cell Lysate, Biological Fluids 3-200 pg/mL 3 pg/mL Log in to see
0.0012117105 ABIN2086395 Sandwich ELISA Log in to see
0.0012117105 ABIN2086397 Sandwich ELISA Log in to see
0.0012117105 ABIN2950647 31.2-2000 pg/mL 31.2 pg/mL Log in to see

Top referenced HBEGF ELISA Kit for ELISA

  • Human HBEGF ELISA Kit for Sandwich ELISA - ABIN625303 (2 Publications): Koob, Lim, Massee, Zabek, Rennert, Gurtner, Li: Angiogenic properties of dehydrated human amnion/chorion allografts: therapeutic potential for soft tissue repair and regeneration. in Vascular cell 2014 (PubMed)

  • Human HBEGF ELISA Kit for Sandwich ELISA - ABIN2684422 (1 Publications): Moschella, Torelli, Valentini, Urbani, Buccione, Petrucci, Natalino, Belardelli, Foà, Proietti: Cyclophosphamide induces a type I interferon-associated sterile inflammatory response signature in cancer patients' blood cells: implications for cancer chemoimmunotherapy. in Clinical cancer research : an official journal of the American Association for Cancer Research 2013 (PubMed)

  • Human HBEGF ELISA Kit for Sandwich ELISA - ABIN1672797 (1 Publications): Fen, Dhadly, Yoshizumi, Hilkert, Quertermous, Eddy, Shows, Lee: Structural organization and chromosomal assignment of the gene encoding the human heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor. in Biochemistry 1993 (PubMed)

Similar HBEGF ELISA Kits

Application / Reactivity Human
ELISA 18 ELISA Kits

General

Antigen Heparin-Binding EGF-Like Growth Factor (HBEGF) ELISA Kits
Epitope AA 63-148
Reactivity Human
Kits with alternative reactivity to:
(19), (13), (13), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 15.6-1000 pg/mL
Minimum Detection Limit 15.6 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details HBEGF ELISA Kit

Target details Application Details Handling References for HBEGF Kit (ABIN1672797) Images
Purpose For quantitative detection of human HBEGF in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human HBEGF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human HBEGF antibod - 1
  • Lyophilized recombinant human HBEGF standar - 10ng/tubex2
  • Biotinylated anti- human HBEGF antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: sf21
Immunogen sequence: D63-L148

Target details

Product Details HBEGF ELISA Kit Application Details Handling References for HBEGF Kit (ABIN1672797) Images back to top
Antigen
Alternative Name HBEGF (HBEGF ELISA Kit Abstract)
Background HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.
Gene ID 1839
UniProt Q99075
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details HBEGF ELISA Kit Target details Handling References for HBEGF Kit (ABIN1672797) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human HBEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HBEGF has been precoated onto 96-well plates. Standards(sf21, D63-L148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HBEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HBEGF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human HBEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HBEGF standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 92.7, Standard deviation: 5.10, CV(%): 5.5
  • Sample 2: n=16, Mean(pg/ml): 369, Standard deviation: 16.97, CV(%): 4.6
  • Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 23.6, CV(%): 3.8,
  • Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 8.26, CV(%): 8.1
  • Sample 2: n=24, Mean(pg/ml): 376, Standard deviation: 27.82, CV(%): 7.4
  • Sample 3: n=24, Mean(pg/ml): 618, Standard deviation: 42.02, CV(%): 6.8
Restrictions For Research Use only

Handling

Product Details HBEGF ELISA Kit Target details Application Details References for HBEGF Kit (ABIN1672797) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for HBEGF Kit (ABIN1672797)

Product Details HBEGF ELISA Kit Target details Application Details Handling Images back to top
Background publications

Fen, Dhadly, Yoshizumi, Hilkert, Quertermous, Eddy, Shows, Lee: "Structural organization and chromosomal assignment of the gene encoding the human heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor." in: Biochemistry, Vol. 32, Issue 31, pp. 7932-8, 1993

Images

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Supplier Images
ELISA image for HBEGF ELISA Kit (Heparin-Binding EGF-Like Growth Factor) (ABIN1672797) Human HBEGF PicoKine ELISA Kit standard curve