|Antigen||KIT Ligand (KITLG) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Reactivity||Human, Primate Alternatives|
|Conjugate||This KIT Ligand antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-KIT Ligand AntibodyTarget Details KIT Ligand Application Details Handling ProductDetails: References for anti-KIT Ligand antibody (ABIN112014) Images
|Specificity||This Monoclonal antibody hKL12 is specific for Stem Cell Factor (hSCF). hKL12 is useful for the detection of hSCF in tissues, isolated cells and for studying biological effects of Human Stem Cell Factor in vitro. Antigen distribution on Tissue sections: Bone marrow stromal cells, fibroblasts, and fetal liver cells express SCF. Keratinocytes stain positive.|
Species reactivity (expected):Primates.
Species reactivity (tested):Human.
|Immunogen||Recombinant Human stem cell factor. Remarks: Recombinant Human SFC is an 18.4 kDa polypeptide containing 165 amino acid residues, corresponding to the sequence of the soluble form of SCF. The antigen is a 248 residue protein. The full-length transcript of the corresponding gene product includes a 25-amino acid secretory leader, a 189-amino acid extracellular domain, a 23-amino acid transmembrane region, and a 36-amino acid cytoplasmic tail. The epitope has not been further characterized.|
Target Details KIT LigandProduct Details anti-KIT Ligand Antibody Application Details Handling ProductDetails: References for anti-KIT Ligand antibody (ABIN112014) Images back to top
|Alternative Name||KITLG / SCF (KITLG Antibody Abstract)|
|Background||SCF is a transmembrane protein expressed as two alternatively spliced isoforms, either 220 or 248 amino acids, the former lacking a peptidase cleavage site. The cleavage site is responsible for the release of the soluble form of the protein from the cell surface. The first 164 or 165 residues of the extracellular domain comprise soluble SCF, which exists in solution as a noncovalently linked homodimer. Alone, SCF is a weakly proliferative stimulus but acts synergistically with numerous cytokines. SCF is important in germ cell developement, and an indispensable facor for mast cell proliferation an differentiation.Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling ProductDetails: References for anti-KIT Ligand antibody (ABIN112014) Images back to top
ELISA (0.15 μg/mL). Has been described to work in Wetsern blots and Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details ProductDetails: References for anti-KIT Ligand antibody (ABIN112014) Images back to top
|Reconstitution||Restore by adding 0.5 mL distilled water.|
|Buffer||PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.|
ProductDetails: References for anti-KIT Ligand antibody (ABIN112014)Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Handling Images back to top
Dippel, Haas, Grabbe, Schadendorf, Hamann, Czarnetzki: "Expression of the c-kit receptor in hypomelanosis: a comparative study between piebaldism, naevus depigmentosus and vitiligo." in: The British journal of dermatology, Vol. 132, Issue 2, pp. 182-9, 1995