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|Antigen||KIT Ligand (KITLG) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This KIT Ligand antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Neutralization (Neut), Western Blotting (WB)
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Product Details anti-KIT Ligand AntibodyTarget Details KIT Ligand Application Details Handling Images
|Specificity||Recognizes Rat SCF|
|Purification||Affinity Chromatography employing an immobilized Rat SCF matrix.|
|Immunogen||Highly pure (>98%) recombinant Rat SCF.|
Target Details KIT LigandProduct Details anti-KIT Ligand Antibody Application Details Handling Images back to top
|Alternative Name||KITLG / SCF (KITLG Antibody Abstract)|
|Background||Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of ratSCF(15 ng/mL), a concentration of 0.08-0.12 μg/mL of this antibody is required. ELISA: To detect rat SCF by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of0.2-0.4 ng/well of recombinant rat SCF. Western Blot: To detect rat SCF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat SCF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Images back to top
|Reconstitution||Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|