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anti-Human CBL antibody for ELISA

Recommended CBL Antibody (supplied by: Log in to see )

Antigen
Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies
  • 4732447J05Rik
  • C-CBL
  • c-Cbl
  • c-cbl-like
  • cbl
  • Cbl-2
  • CBL2
  • FRA11B
  • NSLL
  • RGD1561386
  • RNF55
Reactivity
Human, Mouse (Murine), Rat (Rattus)
250
136
129
6
4
4
4
1
1
1
Host
Mouse
203
35
17
1
Clonality
Monoclonal
Conjugate
This CBL antibody is un-conjugated
7
7
7
7
6
5
5
5
5
5
5
5
4
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
170
71
67
55
55
33
18
16
5
5
4
3
3
1
1
1
1
1
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Catalog No. ABIN968993
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32693866 ABIN560198 IP ELISA WB Mouse IgG1 kappa AA 736-835, partial Log in to see 6D12
0.32693866 ABIN1845278 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0012117105 ABIN1531307 IF IHC ELISA WB Rabbit IgG pTyr700 Log in to see Polyclonal 1
0.0012117105 ABIN1531308 IHC ELISA WB Rabbit IgG pTyr674 Log in to see Polyclonal
0.0012117105 ABIN1531306 IF IHC ELISA WB Rabbit IgG pTyr774 Log in to see Polyclonal
0.0012117105 ABIN1532325 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1532326 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1532327 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN292894 ELISA WB Goat C-Term Log in to see Polyclonal
0.0012117105 ABIN342872 ELISA WB Rabbit pTyr700 Log in to see Polyclonal
0.0012117105 ABIN2888650 IF IHC (p) ELISA WB Rabbit IgG AA 740-789 Log in to see Polyclonal
0.0012117105 ABIN2888651 IF IHC (p) ELISA WB Rabbit IgG AA 666-715 Log in to see Polyclonal
0.0012117105 ABIN2888652 IHC (p) ELISA WB Rabbit IgG AA 640-689 Log in to see Polyclonal
0.0012117105 ABIN2163327 IHC ELISA WB Rabbit IgG Tyr674 Log in to see Polyclonal
0.0012117105 ABIN791051 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0012117105 ABIN514072 ELISA WB Mouse IgG1 kappa AA 736-835, partial Log in to see 6D12
0.0012117105 ABIN514073 ELISA WB Mouse IgG1 kappa AA 736-835, partial Log in to see 6D12
0.0012117105 ABIN1001990 FM ELISA WB Rabbit IgG Log in to see Polyclonal 3
0.0012117105 ABIN560197 ELISA WB Mouse AA 736-835, partial Log in to see Polyclonal
0.0012117105 ABIN2590840 ELISA Mouse IgG1 Log in to see 3B12

Top referenced anti-CBL antibody for ELISA

Similar anti-CBL Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 31 Antibodies 35 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies 4 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 27 Antibodies 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 75 Antibodies

General

Antigen Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(250), (136), (129), (6), (4), (4), (4), (1), (1), (1)
Host Mouse
(203), (35), (17), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CBL antibody is un-conjugated
(7), (7), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(170), (71), (67), (55), (55), (33), (18), (16), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CBL Antibody

Target Details CBL Application Details Handling References for anti-CBL Antibody (ABIN968993) Images
Purification Ascites
Immunogen Purified recombinant fragment of human C-CBL expressed in E. coli.
Clone 3B12
Isotype IgG1

Target Details CBL

Product Details anti-CBL Antibody Application Details Handling References for anti-CBL Antibody (ABIN968993) Images back to top
Antigen
Alternative Name C-CBL (CBL Antibody Abstract)
Background The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
Molecular Weight 120 kDa
Gene ID 867
HGNC 867
Pathways TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CBL Antibody Target Details CBL Handling References for anti-CBL Antibody (ABIN968993) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-CBL Antibody Target Details CBL Application Details References for anti-CBL Antibody (ABIN968993) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-CBL Antibody (ABIN968993)

Product Details anti-CBL Antibody Target Details CBL Application Details Handling Images back to top
Product cited in:

Sproul, Xu, Wilhelm, Gire, Greene: "Cbl negatively regulates JNK activation and cell death." in: Cell research, Vol. 19, Issue 8, pp. 950-61, 2009

Sanada, Suzuki, Shih, Otsu, Kato, Yamazaki, Tamura, Honda, Sakata-Yanagimoto, Kumano, Oda, Yamagata, Takita, Gotoh, Nakazaki, Kawamata, Onodera, Nobuyoshi, Hayashi, Harada, Kurokawa, Chiba, Mori et al.: "Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms. ..." in: Nature, Vol. 460, Issue 7257, pp. 904-8, 2009

Loh, Sakai, Flotho, Kang, Fliegauf, Archambeault, Mullighan, Chen, Bergstraesser, Bueso-Ramos, Emanuel, Hasle, Issa, van den Heuvel-Eibrink, Locatelli, Stary, Trebo, Wlodarski, Zecca, Shannon et al.: "Mutations in CBL occur frequently in juvenile myelomonocytic leukemia. ..." in: Blood, Vol. 114, Issue 9, pp. 1859-63, 2009

Images

Product Details anti-CBL Antibody Target Details CBL Application Details Handling References for anti-CBL Antibody (ABIN968993) back to top
Supplier Images
ELISA image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 2: Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and ne...
Immunocytochemistry (ICC) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 3: Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues us...
Immunohistochemistry (IHC) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues us...
Western Blotting (WB) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 6: Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2),...
Western Blotting (WB) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 7: Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) rec...