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anti-Human CBL antibody for Immunofluorescence

Recommended CBL Antibody (supplied by: Log in to see )

Antigen
Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies
  • 4732447J05Rik
  • C-CBL
  • c-Cbl
  • c-cbl-like
  • cbl
  • Cbl-2
  • CBL2
  • FRA11B
  • NSLL
  • RGD1561386
  • RNF55
Epitope
pTyr700
46
39
26
23
17
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
245
131
125
6
4
4
4
1
1
1
Host
Rabbit
198
35
16
1
Clonality
Polyclonal
Conjugate
This CBL antibody is un-conjugated
7
7
7
7
6
5
5
5
5
5
5
5
4
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
164
67
65
55
50
30
18
13
5
5
4
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN1531307
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31787968 ABIN499585 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 1
0.31787968 ABIN967970 IF IP IHC WB Mouse IgG1 AA 595-810 Log in to see 17-c 5
0.31787968 ABIN967971 IF IP IHC WB Mouse IgG1 AA 595-810 Log in to see 17-c 5
0.31787968 ABIN968811 IF WB Mouse IgG1 pTyr700 Log in to see 47-c 2
0.31787968 ABIN968810 IF WB Mouse IgG1 pTyr700 Log in to see 47-c 2
0.31787968 ABIN1531306 IF IHC ELISA WB Rabbit IgG pTyr774 Log in to see Polyclonal
0.31787968 ABIN1532325 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.31787968 ABIN1532326 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.31787968 ABIN1105707 EIA IF IHC (p) WB Mouse IgG1 Log in to see 3B12 3
0.31787968 ABIN2704546 IC IF IHC WB Rabbit C-Term, pTyr774 Log in to see Polyclonal
0.31787968 ABIN2704545 IC IF IHC WB Rabbit C-Term, pTyr700 Log in to see Polyclonal
0.31787968 ABIN2855019 ICC IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.31787968 ABIN1870034 IF IHC WB Rabbit IgG pTyr700 Log in to see Polyclonal
0.31787968 ABIN2425117 IF IHC WB Rabbit IgG pTyr700 Log in to see Polyclonal
0.31787968 ABIN2156148 IF ELISA WB Rabbit C-Term Log in to see Polyclonal
0.31787968 ABIN1030323 IF ELISA WB Rabbit C-Term Log in to see Polyclonal
0.31787968 ABIN3020049 IF IHC WB Rabbit pTyr700 Log in to see Polyclonal
0.0010833679 ABIN319293 IF IHC (p) WB Rabbit pTyr700 Log in to see Polyclonal 4
0.0010833679 ABIN272004 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010833679 ABIN271925 IF IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-CBL antibody for Immunofluorescence

Similar anti-CBL Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 30 Antibodies 34 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies 3 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 3 Antibodies
Immunofluorescence (IF) 24 Antibodies 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 55 Antibodies
Immunohistochemistry (IHC) 34 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 72 Antibodies

General

Antigen Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) Antibodies
Epitope pTyr700
(46), (39), (26), (23), (17), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(245), (131), (125), (6), (4), (4), (4), (1), (1), (1)
Host Rabbit
(198), (35), (16), (1)
Clonality
Polyclonal
Conjugate This CBL antibody is un-conjugated
(7), (7), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(164), (67), (65), (55), (50), (30), (18), (13), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-CBL Antibody

Target Details CBL Application Details Handling References for anti-CBL Antibody (ABIN1531307) Images
Specificity CBL (Phospho-Tyr700) Antibody detects endogenous levels of CBL only when phosphorylated at Tyr700.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human CBL around the phosphorylation site of Tyr700.
Isotype IgG

Target Details CBL

Product Details anti-CBL Antibody Application Details Handling References for anti-CBL Antibody (ABIN1531307) Images back to top
Antigen
Alternative Name CBL (CBL Antibody Abstract)
Background Molecular weight of target protein: 99 kDa
Synonyms: Cas-Br-M (murine) ecotropic retroviral transforming, CBL E3 ubiquitin protein ligase, CBL2, Proto-oncogene c-CBL, Signal transduction protein CBL
Molecular Weight 99 kDa
Gene ID 867
OMIM 165360
UniProt P22681
Research Area Cancer, Transcription Factors, Proteolysis / Ubiquitin
Pathways TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway

Application Details

Product Details anti-CBL Antibody Target Details CBL Handling References for anti-CBL Antibody (ABIN1531307) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.504096 (NCBI Gene Symbol: CBL)

Restrictions For Research Use only

Handling

Product Details anti-CBL Antibody Target Details CBL Application Details References for anti-CBL Antibody (ABIN1531307) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CBL Antibody (ABIN1531307)

Product Details anti-CBL Antibody Target Details CBL Application Details Handling Images back to top
Background publications

Joazeiro, Wing, Huang, Leverson, Hunter, Liu: "The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase." in: Science (New York, N.Y.), Vol. 286, Issue 5438, pp. 309-12, 1999

Images

Product Details anti-CBL Antibody Target Details CBL Application Details Handling References for anti-CBL Antibody (ABIN1531307) back to top
Supplier Images
Western Blotting (WB) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (pTyr700) (ABIN1531307) Western blot analysis of extracts from K562 cells treated with Na3VO4 0.3nM, using CB...
Immunohistochemistry (IHC) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (pTyr700) (ABIN1531307) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CBL ...
Immunofluorescence (IF) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (pTyr700) (ABIN1531307) Immunofluorescence analysis of HepG2 cells, using CBL (Phospho-Tyr700) Antibody. The ...