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anti-Mouse (Murine) CD247 antibody for Enzyme Immunoassay

Recommended CD247 Antibody (supplied by: Log in to see )

Antigen
CD247 Molecule (CD247) Antibodies
  • 4930549J05Rik
  • A430104F18Rik
  • AW552088
  • Cd3
  • Cd3-eta
  • CD3-ZETA
  • Cd3-zeta
  • CD3H
  • Cd3h
  • CD3Q
  • Cd3z
  • CD3Z
  • Cd3zeta
  • CD247
  • im:7156386
  • LOC100136514
  • LOC100136549
  • LOC100305023
  • T3Z
  • T3z
  • Tcrk
  • Tcrz
  • TCRZ
  • TCRzeta
Reactivity
Human, Mouse (Murine)
267
119
29
4
2
2
1
1
Host
Mouse
155
99
14
11
1
Clonality
Monoclonal
Conjugate
This CD247 antibody is un-conjugated
29
20
10
7
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
198
136
107
57
47
44
34
21
11
7
3
2
1
1
Supplier
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Catalog No. ABIN118361
$ 588.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870064 ABIN501184 EIA FACS IHC (fro) IP WB Mouse IgG2a Cytoplasmic Domain Log in to see G3 1
0.0008870064 ABIN501183 EIA FACS IHC (fro) IP WB Mouse IgG2a Cytoplasmic Domain Log in to see G3 1
0.0008870064 ABIN181785 EIA FACS IHC (fro) IP WB Mouse IgG2a Log in to see G3 1
0.0008870064 ABIN951241 EIA FACS IF WB Hamster IgG Log in to see H146-968

Top referenced anti-CD247 antibody for Enzyme Immunoassay

Similar anti-CD247 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 21 Antibodies 98 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 8 Antibodies
Flow Cytometry (FACS) 48 Antibodies 129 Antibodies
Immunocytochemistry (ICC) 22 Antibodies 47 Antibodies
Immunofluorescence (IF) 21 Antibodies 59 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 108 Antibodies
Functional Studies (Func) 2 Antibodies

General

Antigen CD247 Molecule (CD247) Antibodies
Reactivity Human, Mouse (Murine)
(267), (119), (29), (4), (2), (2), (1), (1)
Host Mouse
(155), (99), (14), (11), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD247 antibody is un-conjugated
(29), (20), (10), (7), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(198), (136), (107), (57), (47), (44), (34), (21), (11), (7), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CD247 Antibody

Target Details CD247 Application Details Handling References for anti-CD247 Antibody (ABIN118361) Images
Specificity A novel monoclonal antibody, named G3, has been developed that is specific for the TCR zeta chain subunit (CD247). The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain.
Purification Affinity chromatography on Protein A
Immunogen 113 Amino acid synthetic peptide corresponding to the cytoplasmatic domain of murine zeta chain. Spleen cells from immunised BALB/c mice were fused with cells of the Ag8.653 mouse myeloma cell line.
Clone G3
Isotype IgG2a

Target Details CD247

Product Details anti-CD247 Antibody Application Details Handling References for anti-CD247 Antibody (ABIN118361) Images back to top
Antigen
Alternative Name CD247 / CD3Z (CD247 Antibody Abstract)
Background The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events.Synonyms: CD3 zeta chain, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ
Gene ID 12503
NCBI Accession NP_001106862
UniProt P24161
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD247 Antibody Target Details CD247 Handling References for anti-CD247 Antibody (ABIN118361) Images back to top
Application Notes Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 106 cells, Membrane permeabilisation is required. Immunoprecipitation: 2,0 μg/mL. ELISA: 1,0 μg/mL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD247 Antibody Target Details CD247 Application Details References for anti-CD247 Antibody (ABIN118361) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD247 Antibody (ABIN118361)

Product Details anti-CD247 Antibody Target Details CD247 Application Details Handling Images back to top
Background publications

van Oers, Teh, Irving, Tiong, Weiss, Teh: "Production and characterization of monoclonal antibodies specific for the murine T cell receptor zeta chain." in: Journal of immunological methods, Vol. 170, Issue 2, pp. 261-8, 1994