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anti-Human CD3E antibody for Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Reactivity
Human
524
412
124
90
65
45
44
43
43
43
30
26
25
24
22
22
21
7
6
2
2
2
2
1
1
Host
Rat
360
225
211
78
35
21
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
97
82
70
40
20
20
19
19
17
16
16
15
14
13
13
13
13
13
13
13
11
11
7
7
6
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Flow Cytometry (FACS), Western Blotting (WB)
691
297
218
168
128
92
88
85
85
34
25
9
9
6
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2166654
$ 957.14
Plus shipping costs $45.00

Similar anti-CD3E Antibodies

Application / Reactivity Human
Activation (Act) 1 Antibodies
Agonist (Agon) 1 Antibodies
Blocking Reagent (BR) 3 Antibodies
ELISA 86 Antibodies
Flow Cytometry (FACS) 225 Antibodies
Functional Studies (Func) 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 85 Antibodies
Immunofluorescence (IF) 97 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 82 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 4 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 57 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 69 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Human
(524), (412), (124), (90), (65), (45), (44), (43), (43), (43), (30), (26), (25), (24), (22), (22), (21), (7), (6), (2), (2), (2), (2), (1), (1)
Host Rat
(360), (225), (211), (78), (35), (21), (1)
Clonality
Monoclonal
Conjugate This CD3E antibody is un-conjugated
(97), (82), (70), (40), (20), (20), (19), (19), (17), (16), (16), (15), (14), (13), (13), (13), (13), (13), (13), (13), (11), (11), (7), (7), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Flow Cytometry (FACS), Western Blotting (WB)
(691), (297), (218), (168), (128), (92), (88), (85), (85), (34), (25), (9), (9), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Specificity Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3epsilon chain recognizes human CD3epsilon. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (epsilon, lamda, delta, zeta) that assemble and function as three pairs of dimers (epsilonlamda, epsilondelta, zetazeta). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008, Guy and Vignali 2009, Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993, Kothlow et al. 2005)
Predicted Reactivity Human
Characteristics Rat Anti Human Cd3
Protein Size: 207
Immunogen The immunogen for anti-CD3E antibody: synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys)
Isotype IgG1

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3E (CD3E Antibody Abstract)
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes Flow Cytometry(1): 1/50 - 1/100. Immunohistology - Frozen: 1/100. Immunohistology - Paraffin(2): 1/100.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Format Liquid
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2166654) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2166654) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (Image 2)
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (ABIN2166654) anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (Image 3)