anti-Mouse (Murine) CD3E antibody for Immunohistochemistry (Zinc-fixed Sections)

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Mouse (Murine)
512
482
122
88
53
34
29
29
28
27
21
16
15
12
9
8
6
5
5
5
2
2
2
1
1
1
Host
Armenian Hamster
463
232
200
63
21
15
4
1
1
Clonality
Monoclonal
Conjugate
This CD3E antibody is un-conjugated
96
79
76
37
25
22
21
20
20
18
17
17
17
14
14
14
14
12
12
12
12
10
8
8
7
7
6
5
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP), Western Blotting (WB)
728
343
243
203
152
147
113
99
99
42
30
25
13
8
8
7
6
4
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN2689215
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General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Mouse (Murine)
(512), (482), (122), (88), (53), (34), (29), (29), (28), (27), (21), (16), (15), (12), (9), (8), (6), (5), (5), (5), (2), (2), (2), (1), (1), (1)
Host Armenian Hamster
(463), (232), (200), (63), (21), (15), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD3E antibody is un-conjugated
(96), (79), (76), (37), (25), (22), (21), (20), (20), (18), (17), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (10), (8), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP), Western Blotting (WB)
(728), (343), (243), (203), (152), (147), (113), (99), (99), (42), (30), (25), (13), (8), (8), (7), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling ProductDetails: References for anti-CD3E antibody (ABIN2689215) Images
Brand BD Pharmingen™
Characteristics The 145-2C11 antibody reacts with the 25- kDa ε chain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells. The cytoplasmic domain of CD3e participates in the signal transduction events which activate several cellular biochemical pathways as a result of antigen recognition. Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro, in the presence of Fc receptor-bearing accessory cells. In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells. Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's. Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death. The 145-2C11 antibody does not cross-react with rat leukocytes and it has been reported that pre-incubation of thymus cell suspensions at 37 °C for 2-4 hours prior to staining enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes. Immunohistochemical staining of T lymphocytes. The frozen section of normal mouse spleen was reacted with 145-2C11 mAb. The positive staining is represented by the brown labeling of cell-surface membranes of lymphocytes in the periarteriolar sheath.

BD Pharmingen™ Purified Hamster Anti-Mouse CD3e - Purified - Clone 145-2C11 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 1 mL
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Clone 145
Isotype IgG1, kappa

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling ProductDetails: References for anti-CD3E antibody (ABIN2689215) Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background Synonyms: CD3ε chain
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling ProductDetails: References for anti-CD3E antibody (ABIN2689215) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details ProductDetails: References for anti-CD3E antibody (ABIN2689215) Images back to top
Format Liquid
Concentration 31.25 μg/mL
Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

ProductDetails: References for anti-CD3E antibody (ABIN2689215)

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling Images back to top
Product cited in:

Castro, Listman, Jacobson, Wang, Lopez, Ju, Finn, Perkins: "Fas modulation of apoptosis during negative selection of thymocytes." in: Immunity, Vol. 5, Issue 6, pp. 617-27, 1997

Nakano, Yamazaki, Miyatake, Nozaki, Kikuchi, Saito: "Specific interaction of topoisomerase II beta and the CD3 epsilon chain of the T cell receptor complex." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6483-9, 1996

Shinkai, Alt: "CD3 epsilon-mediated signals rescue the development of CD4+CD8+ thymocytes in RAG-2-/- mice in the absence of TCR beta chain expression." in: International immunology, Vol. 6, Issue 7, pp. 995-1001, 1994

Salvadori, Gansbacher, Pizzimenti, Zier: "Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 5176-82, 1994

Leo, Foo, Sachs, Samelson, Bluestone: "Identification of a monoclonal antibody specific for a murine T3 polypeptide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1374-8, 1987