anti-Pig (Porcine) CD3E antibody for Western Blotting

Recommended CD3E Antibody (supplied by: Log in to see )

Antigen
CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
  • CD3 epsilon
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3 antigen, epsilon polypeptide
  • CD3 molecule, epsilon
  • cd3e
  • CD3E
  • Cd3e
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Cryptomys amatus (Mole rat), Dog (Canine), Elephant, Frog, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Opossum (Didelphimorphia), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine)
543
486
121
87
52
33
28
28
27
26
20
16
14
11
8
7
6
5
5
5
1
1
1
1
1
1
Host
Rabbit
494
232
203
64
22
15
4
1
1
Clonality
Polyclonal
Conjugate
This CD3E antibody is un-conjugated
96
81
77
39
28
24
23
20
20
20
19
17
17
14
14
14
14
12
12
12
12
10
10
9
9
8
6
5
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
758
346
245
204
156
147
120
102
100
42
30
27
13
9
9
7
6
4
3
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN3026772
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2561723 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.2182703 ABIN4291521 FACS ICC IF IP WB Rabbit Log in to see Polyclonal
1 ABIN440186 FACS IHC IHC (fro) IHC (p) IP in vitro WB FITC Mouse IgG1 kappa Log in to see PPT3
1 ABIN440187 FACS IHC IHC (fro) IHC (p) IP in vitro WB Biotin Mouse IgG1 kappa Log in to see PPT3
1 ABIN5608850 WB Goat IgG Log in to see Polyclonal
1 ABIN440185 FACS IHC IHC (fro) IHC (p) IP in vitro WB Mouse IgG1 kappa Log in to see PPT3
1 ABIN4291519 FACS ICC IF IP WB Rabbit Log in to see Polyclonal
1 ABIN4291565 IHC WB Mouse IgG1 Log in to see PC3-188A
1 ABIN4957815 FACS IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see PPT3
1 ABIN3026773 FACS IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN2739341 FACS IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN4291520 FACS ICC IF IP WB Rabbit Log in to see Polyclonal
1 ABIN4291566 IHC WB Alexa Fluor 405 Mouse IgG1 Log in to see PC3-188A
1 ABIN4291567 IHC WB Alexa Fluor 488 Mouse IgG1 Log in to see PC3-188A
1 ABIN4291569 IHC WB Alexa Fluor 700 Mouse IgG1 Log in to see PC3-188A
1 ABIN4291575 IHC WB DyLight 488 Mouse IgG1 Log in to see PC3-188A
1 ABIN4291578 WB PerCP Mouse IgG1 Log in to see PC3-188A
1 ABIN4291571 IHC WB Biotin Mouse IgG1 Log in to see PC3-188A
1 ABIN4291577 IHC WB DyLight 755 Mouse IgG1 Log in to see PC3-188A
1 ABIN4291572 IHC WB DyLight 650 Mouse IgG1 Log in to see PC3-188A

General

Antigen CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Cryptomys amatus (Mole rat), Dog (Canine), Elephant, Frog, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Opossum (Didelphimorphia), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine)
(543), (486), (121), (87), (52), (33), (28), (28), (27), (26), (20), (16), (14), (11), (8), (7), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1)
Host Rabbit
(494), (232), (203), (64), (22), (15), (4), (1), (1)
Clonality
Polyclonal
Conjugate This CD3E antibody is un-conjugated
(96), (81), (77), (39), (28), (24), (23), (20), (20), (20), (19), (17), (17), (14), (14), (14), (14), (12), (12), (12), (12), (10), (10), (9), (9), (8), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
(758), (346), (245), (204), (156), (147), (120), (102), (100), (42), (30), (27), (13), (9), (9), (7), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD3E Antibody

Target Details CD3E Application Details Handling Images
Characteristics This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
Purification Protein A purified CD3e antibody
Immunogen Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
Isotype IgG

Target Details CD3E

Product Details anti-CD3E Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD3e (CD3E Antibody Abstract)
Background This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
Gene ID 916
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD3E Antibody Target Details CD3E Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.\. FACS: use the CD3e antibody at 0.5-1 μg/million cells,WB: 0.5-1.0 μg/mL,IP: 1-2 μg/500 μg protein lysate,
Restrictions For Research Use only

Handling

Product Details anti-CD3E Antibody Target Details CD3E Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-CD3E Antibody Target Details CD3E Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN3026772) Western blot testing of human Jurkat lysate in the absence and presence of immunizing...
Western Blotting (WB) image for anti-CD3e Molecule, epsilon (CD3-TCR Complex) (CD3E) antibody (ABIN3026772) Western blot testing of Jurkat lysate with CD3e antibody.