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anti-Mouse (Murine) LHB antibody for Immunohistochemistry (Frozen Sections)

Recommended LHB Antibody (supplied by: Log in to see )

Antigen
Luteinizing Hormone beta Polypeptide (LHB) Antibodies
  • cgb4
  • CGB4
  • hlhb
  • hLHB
  • LH
  • LH-B
  • lhb-A
  • LSH-B
  • lsh-b
  • LSH-beta
  • xlhb
Epitope
AA 21-40, N-Term
4
4
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
135
12
9
7
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
108
42
3
1
Clonality
Polyclonal
Conjugate
This LHB antibody is un-conjugated
10
8
7
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
59
50
43
30
15
11
10
10
6
4
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN493089
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.000883884 ABIN493091 DB EIA IHC (fro) Rabbit AA 115-134, C-Term Log in to see Polyclonal 1

Top referenced anti-LHB antibody for Immunohistochemistry (Frozen Sections)

Similar anti-LHB Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
Dot Blot (DB) 2 Antibodies 2 Antibodies 2 Antibodies
ELISA 6 Antibodies 3 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Western Blotting (WB) 6 Antibodies 5 Antibodies
Detection (D) 1 Antibodies
ELISA (Detection) 1 Antibodies

General

Antigen Luteinizing Hormone beta Polypeptide (LHB) Antibodies
Epitope AA 21-40, N-Term
(4), (4), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(135), (12), (9), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(108), (42), (3), (1)
Clonality
Polyclonal
Conjugate This LHB antibody is un-conjugated
(10), (8), (7), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
(59), (50), (43), (30), (15), (11), (10), (10), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-LHB Antibody

Target Details LHB Application Details Handling References for anti-LHB Antibody (ABIN493089) Images
Sequence RPW CHP INA ILA VEK EGC PV
Specificity This antibody labels gonadotrophs in pituitary sections. It does not react to other cellular proteins that are tested during characterization of this antibody.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat.
Purification Affinity Chromatography.
Immunogen Synthetic peptide corresponding to 21-40 amino acids of Human LH

Target Details LHB

Product Details anti-LHB Antibody Application Details Handling References for anti-LHB Antibody (ABIN493089) Images back to top
Antigen
Alternative Name Luteinizing Hormone beta (LSH-Beta) (LHB Antibody Abstract)
Background The pituitary hormones, luteinizing hormone (lutropin, LH) and follicle stimulating hormone (follitropin, FSH) as well as related placental hormones, chorionic gonadotropin and CG, are referred to as gonadotropic hormones because of their action on gonad cells. These three hormones are structurally related to each other as well as to thyroid stimulating hormone (TSH). Because of their similar structure and composition and glycoprotein nature, LH, TSH, GC and FSH, these hormones are referred to as glycoprotein hormones. All these hormones are heterodimeric in nature composed of two un-identical subunits, alpha and beta which are non-covalently associated, Among humans and other species, the Alpha subunit is identical for all these hormones and is coded by a single gene, where is beta subunits are distinct but homologous and arise from separate genes. The individual hormone subunits and peptides derived from them have shown some binding activities to their respective receptors, the association of alpha and beta subunits clearly is required for optimal activation and physiological responses elicited by these hormones. Within the heterodimeric hormones, the beta subunit confors the binding activity to its respective receptor. The LH and CG beta subunits have 85 % sequence identity in their first 114 amino acids, the LH/CG receptors can recognize either of the pituitary LH or placental CG hormones. The alpha and beta subunits of LH, CG and FSH are highly glycosylated (1). The alpha subunit has two N-linked oligosdaccharide chain at position 52 and 78 and beta subunits have one in LH and TSH and two in CG and FSH. The CG beta subunit also has an extension of serine O-linked carbohydrates at carboxyl end which help is plasma half like of CG compared to other glycohormone beta subunits. The N-linked oligosaccharides on FSH are sialylated whereas LH and TSH are sialylated and sulfated. There are 10-12 cysteins forming 3 disulfide bonds referred to as Synonyms: LH-B, LHB, LSH-beta, Luteinizing hormone subunit beta, Lutropin beta chain, Lutropin subunit beta
Gene ID 3972
NCBI Accession NP_000885
UniProt P01229
Pathways TCR Signaling

Application Details

Product Details anti-LHB Antibody Target Details LHB Handling References for anti-LHB Antibody (ABIN493089) Images back to top
Application Notes Dot blot. ELISA: 1/50,000 in dilution buffer. Immunohistochemistry on Frozen Sections: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LHB Antibody Target Details LHB Application Details References for anti-LHB Antibody (ABIN493089) Images back to top
Concentration 0.59-0.72 mg/mL
Buffer Stabilization buffer with 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-LHB Antibody (ABIN493089)

Product Details anti-LHB Antibody Target Details LHB Application Details Handling Images back to top
Background publications

Sairam: "Role of carbohydrates in glycoprotein hormone signal transduction." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 3, Issue 8, pp. 1915-26, 1989