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anti-Human SH2B3 antibody for Western Blotting

Recommended SH2B3 Antibody (supplied by: Log in to see )

Antigen
SH2B Adaptor Protein 3 (SH2B3) Antibodies
  • LNK
  • IDDM20
  • AI429800
  • Lnk
  • SH2B adaptor protein 3
  • SH2B3
  • Sh2b3
Epitope
N-Term
18
5
3
2
1
1
Reactivity
Human
66
21
19
2
Host
Goat
30
21
17
Clonality
Polyclonal
Conjugate
This SH2B3 antibody is un-conjugated
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
48
17
16
13
13
7
5
4
2
1
1
Supplier
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Available images

Catalog No. ABIN151695
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.022232 ABIN2454598 FACS IF WB Mouse IgG1 Log in to see 2C9
3.022232 ABIN2454470 FACS IF WB Mouse IgG1 Log in to see 1E6
3.022232 ABIN2454462 FACS IF WB Mouse IgG2a Log in to see 2B11
3.022232 ABIN2454696 FACS IF WB Mouse IgG1 Log in to see 1F6
3.022232 ABIN2454461 FACS WB Mouse IgG1 Log in to see 6F11
3.022232 ABIN2454471 FACS IF IHC WB Mouse IgG1 Log in to see 2D8
3.022232 ABIN4331277 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.022232 ABIN2706479 WB Rabbit Center Log in to see Polyclonal
3.022232 ABIN2465907 ELISA WB Goat N-Term Log in to see Polyclonal
3.022232 ABIN1859674 ICC IHC IP WB Rabbit IgG AA 335-561 Log in to see Polyclonal
3.022232 ABIN2475350 WB Goat IgG N-Term Log in to see Polyclonal 4
3.022232 ABIN4331278 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 2D8
3.022232 ABIN2563705 ELISA WB Goat IgG N-Term Log in to see Polyclonal
3.022232 ABIN2444920 WB Goat Log in to see Polyclonal
1 ABIN185272 ELISA WB Goat N-Term Log in to see Polyclonal 1
1 ABIN374458 WB Goat N-Term Log in to see Polyclonal 1
1 ABIN318699 WB Goat IgG N-Term Log in to see Polyclonal 2
1 ABIN1500909 FACS IF WB Mouse IgG1 Log in to see 1F6
1 ABIN1500907 FACS IF WB Mouse IgG1 Log in to see 1E6
1 ABIN1500910 FACS WB Mouse IgG1 Log in to see 6F11

Top referenced anti-SH2B3 antibody for Western Blotting

Similar anti-SH2B3 Antibodies

Application / Reactivity Human
ELISA 17 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 48 Antibodies

General

Antigen SH2B Adaptor Protein 3 (SH2B3) Antibodies
Epitope N-Term
(18), (5), (3), (2), (1), (1)
Reactivity Human
(66), (21), (19), (2)
Host Goat
(30), (21), (17)
Clonality
Polyclonal
Conjugate This SH2B3 antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(48), (17), (16), (13), (13), (7), (5), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-SH2B3 Antibody

Target Details SH2B3 Application Details Handling References for anti-SH2B3 Antibody (ABIN151695) Images
Purification Immunogen affinity purified
Immunogen Peptide with sequence NGPALQPSSPC corresponding to N-Terminus according to NP_005466.1.

Target Details SH2B3

Product Details anti-SH2B3 Antibody Application Details Handling References for anti-SH2B3 Antibody (ABIN151695) Images back to top
Antigen
Alternative Name LNK/SH2B3 (SH2B3 Antibody Abstract)
Background Gene Symbol: SH2B3
Gene ID 10019
Pathways TCR Signaling

Application Details

Product Details anti-SH2B3 Antibody Target Details SH2B3 Handling References for anti-SH2B3 Antibody (ABIN151695) Images back to top
Application Notes Western Blot 0.3 - 10 μg/mL, Peptide ELISA Detection limit 1:64000WB: Approx. 70 kDa band observed in MOLT4 lysates (calculated MW of 63.2 kDa band according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-SH2B3 Antibody Target Details SH2B3 Application Details References for anti-SH2B3 Antibody (ABIN151695) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-SH2B3 Antibody (ABIN151695)

Product Details anti-SH2B3 Antibody Target Details SH2B3 Application Details Handling Images back to top
Product cited in:

Gery, Cao, Gueller, Xing, Tefferi, Koeffler: "Lnk inhibits myeloproliferative disorder-associated JAK2 mutant, JAK2V617F." in: Journal of leukocyte biology, Vol. 85, Issue 6, pp. 957-65, 2009

Li, He, Schembri-King, Jakes, Hayashi: "Cloning and characterization of human Lnk, an adaptor protein with pleckstrin homology and Src homology 2 domains that can inhibit T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 10, pp. 5199-206, 2000

Images

Product Details anti-SH2B3 Antibody Target Details SH2B3 Application Details Handling References for anti-SH2B3 Antibody (ABIN151695) back to top
Supplier Images
Western Blotting (WB) image for anti-SH2B3 antibody (SH2B Adaptor Protein 3) (N-Term) (ABIN151695) ABIN151695 (0.3?g/ml) staining of MOLT4 lysate (35?g protein in RIPA buffer).Primary ...
Western Blotting (WB) image for anti-SH2B3 antibody (SH2B Adaptor Protein 3) (N-Term) (ABIN151695) Western Blot: LNK/SH2B3 Antibody [ABIN151695] - ABIN151695 (0.3 ug/ml) staining of MO...
Western Blotting (WB) image for anti-SH2B3 antibody (SH2B Adaptor Protein 3) (N-Term) (ABIN151695) Western Blot: LNK/SH2B3 Antibody [ABIN151695] - HEK293 overexpressing SH2B3 and probe...