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Human ZAP70 ELISA Kit

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Antigen
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) ELISA Kits
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta-chain (TCR) associated protein kinase 70kDa
  • tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase
  • zeta-chain (TCR) associated protein kinase 70
  • zap70
  • ZAP70
  • Smp_149470
  • Zap70
Epitope
pTyr493
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
12
7
2
Method Type
Sandwich ELISA
Application
ELISA
Options
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Catalog No. ABIN2747919
$ 578.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2747920 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN1380282 Cell ELISA Log in to see
1 ABIN1380281 Cell ELISA Log in to see
1 ABIN1380734 Cell ELISA Log in to see
1 ABIN4886351 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN4885072 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate Log in to see
1 ABIN2064841 Cell ELISA Log in to see
1 ABIN2064846 Cell ELISA Log in to see
1 ABIN2064843 Cell ELISA Log in to see
1 ABIN1164645 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2952258 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN5070854 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

Similar ZAP70 ELISA Kits

Application / Reactivity Human
ELISA 13 ELISA Kits

General

Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) ELISA Kits
Epitope pTyr493
Reactivity Human
Kits with alternative reactivity to:
(12), (7), (2)
Method Type Sandwich ELISA
Application ELISA
Supplier Log in to see

Product Details ZAP70 ELISA Kit

Target details Application Details Handling Images
Purpose Human Phospho-ZAP70 (Y493) ELISA Kit. This assay semi-quantitatively measures phophorylated ZAP70 (Tyr493) in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes human ZAP70 phosphorylated at site Tyrosine-493.
Characteristics
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details ZAP70 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name ZAP70 (ZAP70 ELISA Kit Abstract)
Background Zeta-Chain Associated Protein Kinase 70 (ZAP70) phosphorylated at Tyrosine-493
Gene ID 22637
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details ZAP70 ELISA Kit Target details Handling Images back to top
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details ZAP70 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months