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|Application / Reactivity||Human|
|ELISA||14 ELISA Kits|
|Antigen||zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Supplier||Log in to see|
Product Details ZAP70 ELISA KitTarget details Application Details Handling Images
|Purpose||Human Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit. This assay semi-quantitatively measures phophorylated ZAP70 (Tyr493) and Total ZAP70 in lysate samples.|
|Sample Type||Cell Lysate, Tissue Lysate|
|Specificity||The antibody pair provided in this kit recognizes human ZAP70 phosphorylated at site Tyrosine-493 and Total ZAP70.|
|Material not included||
Target detailsProduct Details ZAP70 ELISA Kit Application Details Handling Images back to top
|Alternative Name||ZAP70 (ZAP70 ELISA Kit Abstract)|
|Background||Zeta-chain associated protein kinase 70 (ZAP70) phosphorylated at Tyrosine-493 and Total ZAP70|
|Research Area||Signaling, Tags/Labels, Immunology, Cancer, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details ZAP70 ELISA Kit Target details Handling Images back to top
|Sample Volume||100 μL|
Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
|Restrictions||For Research Use only|
HandlingProduct Details ZAP70 ELISA Kit Target details Application Details Images back to top
|Storage Comment||Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.|
|Expiry Date||6 months|