anti-Rat (Rattus) CAMK2A antibody for Immunocytochemistry

Recommended CAMK2A Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
  • camk2
  • camk2b
  • zgc:112538
  • zgc:123320
  • CAMKA
  • CaMKII
  • R74975
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • CAMK2A
  • camk2a
  • Camk2a
Epitope
pThr286, pThr287
38
30
12
10
9
7
6
6
5
5
4
4
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
146
99
91
28
10
9
6
5
4
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
118
53
18
Clonality
Monoclonal
Conjugate
This CAMK2A antibody is un-conjugated
6
6
6
6
6
5
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
179
86
57
53
43
26
23
7
4
4
2
2
2
1
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Catalog No. ABIN451525
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN151484 ICC IF IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see 6G9 4
1 ABIN227219 ICC IP ELISA WB Mouse IgG1 AA 281-294 Log in to see
1 ABIN1867011 ICC IHC IP WB Rabbit IgG AA 1-478 Log in to see Polyclonal

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) Antibodies
Epitope pThr286, pThr287
(38), (30), (12), (10), (9), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(146), (99), (91), (28), (10), (9), (6), (5), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(118), (53), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CAMK2A antibody is un-conjugated
(6), (6), (6), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(179), (86), (57), (53), (43), (26), (23), (7), (4), (4), (2), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-CAMK2A Antibody

Target Details CAMK2A Application Details Handling References for anti-CAMK2A antibody (ABIN451525) Images
Specificity This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).
Purification Protein G purified
Immunogen Synthetic peptide and it detects phosphorylated CaMKII from rat tissues.
Clone 22B1
Isotype IgG1

Target Details CAMK2A

Product Details anti-CAMK2A Antibody Application Details Handling References for anti-CAMK2A antibody (ABIN451525) Images back to top
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background Gene Symbol: CAMK2A
Gene ID 815
UniProt P11275
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction

Application Details

Product Details anti-CAMK2A Antibody Target Details CAMK2A Handling References for anti-CAMK2A antibody (ABIN451525) Images back to top
Application Notes Western Blot 1 μg/mL, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:5001 μg/mL was sufficient for detection of 0.2 μg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details References for anti-CAMK2A antibody (ABIN451525) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4) and 50 % Glycerol
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CAMK2A antibody (ABIN451525)

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling Images back to top
Product cited in:

Conlon, Thomas, Fedotova, Hernandez-Ono, Di Paolo, Chan, Ruggles, Gibeley, Liu, Ginsberg: "Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis." in: The Journal of clinical investigation, Vol. 126, Issue 10, pp. 3852-3867, 2016

Jiang, Xu, Song, Li, Mao, Zhao, He, Yang, Dai: "Calmodulin-dependent protein kinase II/cAMP response element-binding protein/Wnt/β-catenin signaling cascade regulates angiotensin II-induced podocyte injury and albuminuria." in: The Journal of biological chemistry, Vol. 288, Issue 32, pp. 23368-79, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product Details anti-CAMK2A Antibody Target Details CAMK2A Application Details Handling References for anti-CAMK2A antibody (ABIN451525) back to top
Supplier Images
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [ABIN4515259] - ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)...
Immunohistochemistry (IHC) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [ABIN451...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)...