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|Application / Reactivity||Human||Rat (Rattus)||Mouse (Murine)||Cow (Bovine)||Pig (Porcine)|
|Immunofluorescence (fixed cells) (IF/ICC)||4 Antibodies||3 Antibodies||1 Antibodies|
|Western Blotting (WB)||68 Antibodies||5 Antibodies||13 Antibodies||5 Antibodies||26 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies||12 Antibodies||6 Antibodies||3 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies||30 Antibodies||7 Antibodies||12 Antibodies||2 Antibodies|
|Antigen||Secreted Frizzled-Related Protein 4 (SFRP4) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus) Alternatives|
|Conjugate||This SFRP4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-SFRP4 AntibodyTarget Details SFRP4 Application Details Handling Images
|Specificity||This antibody recognizes SFRP4.|
Species reactivity (expected):Mouse, Rat, Bovine and Porcine.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence TNSSCQCPHILPHQD, from the internal region of the protein sequence|
Target Details SFRP4Product Details anti-SFRP4 Antibody Application Details Handling Images back to top
|Alternative Name||SFRP4 (SFRP4 Antibody Abstract)|
|Background||Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.Synonyms: FRP4, FRPHE, Frizzled protein human endometrium, SFRP-4, Secreted frizzled-related protein 4|
|Gene ID||6424, 9606|
|Research Area||Stem Cells, Wnt, Wnt Signaling|
Application DetailsProduct Details anti-SFRP4 Antibody Target Details SFRP4 Handling Images back to top
Peptide ELISA: Detection limit dilution: 1/16000. Western blot: 0.3-1 μg/mL. Approx.40 kDa band observed in Human Uterus lysates(calculated MW of 39.0 kDa according to NP_003005.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SFRP4 Antibody Target Details SFRP4 Application Details Images back to top
|Buffer||Tris saline, pH 7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-SFRP4 Antibody Target Details SFRP4 Application Details Handling back to top