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anti-Mouse (Murine) TCF4 antibody for Electrophoretic Mobility-Shift Assay

Recommended TCF4 Antibody (supplied by: Log in to see )

Antigen
Transcription Factor 4 (TCF4) Antibodies
  • 5730422P05Rik
  • ASP-I2
  • bHLHb19
  • E2-2
  • e2-A
  • E2.2
  • GE2
  • ITF-2
  • ITF-2b
  • ITF2
  • ME2
  • MITF-2A
  • MITF-2B
  • mTcf-4B
  • mTcf-4E
  • PTHS
  • SEF-2
  • SEF2
  • SEF2-1
  • SEF2-1A
  • SEF2-1B
  • Tcf-4
  • TCF-4
  • tcf-4
  • TCF4
  • Tcf4
  • TCF4B
  • TCF4E
  • TCF7L2
  • TFE
  • XE2
  • Xtcf-4
  • Xtcf4
Epitope
AA 31-331
24
15
5
4
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
82
18
12
4
2
1
1
1
1
Host
Mouse
59
14
9
Clonality
Monoclonal
Conjugate
This TCF4 antibody is un-conjugated
3
3
3
3
3
3
Application
Electrophoretic Mobility-Shift Assay (EMSA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
74
41
21
18
12
12
7
4
2
1
1
Supplier
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Catalog No. ABIN264394
$ 434.50
Plus shipping costs $45.00

Top referenced anti-TCF4 antibody for Electrophoretic Mobility-Shift Assay

Similar anti-TCF4 Antibodies

Application / Reactivity Mouse (Murine) Human
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies 1 Antibodies
ELISA 6 Antibodies 41 Antibodies
Immunochromatography (IC) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 4 Antibodies
Immunofluorescence (IF) 4 Antibodies 21 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 19 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Flow Cytometry (FACS) 18 Antibodies

General

Antigen Transcription Factor 4 (TCF4) Antibodies
Epitope AA 31-331
(24), (15), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(82), (18), (12), (4), (2), (1), (1), (1), (1)
Host Mouse
(59), (14), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TCF4 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Electrophoretic Mobility-Shift Assay (EMSA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(74), (41), (21), (18), (12), (12), (7), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-TCF4 Antibody

Target Details TCF4 Application Details Handling References for anti-TCF4 Antibody (ABIN264394) Images
Characteristics Synonyms: T-Cell Factor-3/4, TCF-3, TCF-4
Purification Purified
Immunogen His-tagged fusion protein corresponding to amino acids 31-331 of the human TCF.
Clone 6F12-3
Isotype IgG1

Target Details TCF4

Product Details anti-TCF4 Antibody Application Details Handling References for anti-TCF4 Antibody (ABIN264394) Images back to top
Antigen
Alternative Name TCF4 (TCF4 Antibody Abstract)
Background TCF-3 and TCF-4 (T cell factor-4) are a members of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF-4 generates transcriptionally active complexes with catenin in colon carcinoma cells. Expression of TCF-4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays an essential role in development. Aberrant TCF-4 transcription activity has been implicated in carcinogenesis of intestinal and mammary epithlia. TCF-3 appears to be expressed exclusively of TCF-4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.Synonyms: E2A, ITF-1, ITF-1, ITF-2, ITF1, ITF1, ITF2, SEF-2, SEF2, TCF-3, TCF-3, TCF-4, TCF-4, Transcription factor 3, Transcription factor E2-alpha
Research Area Stem Cells, Wnt, Transcription Factors, Wnt Signaling
Pathways WNT Signaling

Application Details

Product Details anti-TCF4 Antibody Target Details TCF4 Handling References for anti-TCF4 Antibody (ABIN264394) Images back to top
Application Notes Western Blotting: Detects TCF-4 at 1-4 μg/mL and a 60 kDa band in Cos-1 cells transfectedwith human TCF-3. Immunohistochemistry (Paraffin Section Staining). Gel Shift.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details References for anti-TCF4 Antibody (ABIN264394) Images back to top
Buffer 0.1 M Tris-Glycine, pH 7.4, 150 mM Sodium Chloride, 30 % Glycerol and 0.08 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months

References for anti-TCF4 Antibody (ABIN264394)

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling Images back to top
Product cited in:

Denys, Jadidizadeh, Amini Nik, Van Dam, Aerts, Alman, Cassiman, Tejpar: "Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors." in: Oncogene, Vol. 23, Issue 3, pp. 654-64, 2004

Background publications

Barker, Huls, Korinek, Clevers: "Restricted high level expression of Tcf-4 protein in intestinal and mammary gland epithelium." in: The American journal of pathology, Vol. 154, Issue 1, pp. 29-35, 1999

Images

Product Details anti-TCF4 Antibody Target Details TCF4 Application Details Handling References for anti-TCF4 Antibody (ABIN264394) back to top
Supplier Images
Western Blotting (WB) image for anti-TCF4 antibody (Transcription Factor 4) (AA 31-331) (ABIN264394) anti-Transcription Factor 4 (TCF4) (AA 31-331) antibody